辣椒种间杂种(C. annuum × C. chinense)的获取、鉴定与分析

IF 1.7 4区 农林科学 Q2 HORTICULTURE Journal of Horticultural Science & Biotechnology Pub Date : 2015-11-07 DOI:10.1080/14620316.2015.11513150
Y. H. Shi, N. Hu
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引用次数: 6

摘要

摘要利用胚胎抢救技术,获得了一株母本为紫辣椒(Capsicum annuum 7033)、父本为绿辣椒(c.c ainense 7020)的F1植株。我们使用过氧化物酶同工酶测定和随机扩增多态性DNA (RAPD)标记验证了这种种间杂交的起源。F1植株的大部分形态特征,特别是其叶、花和未成熟果实的紫色,介于其亲本之间。细胞学分析表明,F1植株花粉母细胞的减数分裂染色体表现出异常行为,导致可育花粉比例明显低于亲本。核糖体DNA内转录间隔段的序列分析表明,与亲本序列相比,F1杂种有186个多态性位点,其中139个(74.7%)遗传自父本,23个(12.4%)遗传自母本。
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Acquisition, identification and analysis of an interspecific Capsicum hybrid (C. annuum × C. chinense)
SummaryUsing an embryo rescue technique, we obtained an F1 plantlet whose female parent was a purple chilli pepper (Capsicum annuum 7033) and whose male parent was a green chilli pepper (C. chinense 7020).We verified the origin of this interspecific hybrid using a peroxidase isozyme assay and random amplified polymorphic DNA (RAPD) markers. Most of the morphological features of the F1 plant, particularly the purple colour of its foliage, flowers and immature fruit, were intermediate between those of its parents. Cytological analysis indicated that the meiotic chromosomes of the pollen mother cells of the F1 plant exhibited abnormal behaviour, resulting in a marked reduction in the proportion of fertile pollen compared to both its parents. Sequence analysis of the internal transcribed spacer of nuclear ribosomal DNA demonstrated that, compared to the parental sequences, the F1 hybrid had 186 polymorphic sites, 139 of which (74.7%) were inherited from the male parent, and 23 (12.4%) were inherited from the ...
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来源期刊
CiteScore
3.90
自引率
5.30%
发文量
67
审稿时长
3 months
期刊介绍: The Journal of Horticultural Science and Biotechnology is an international, peer-reviewed journal, which publishes original research contributions into the production, improvement and utilisation of horticultural crops. It aims to provide scientific knowledge of interest to those engaged in scientific research and the practice of horticulture. The scope of the journal includes studies on fruit and other perennial crops, vegetables and ornamentals grown in temperate or tropical regions and their use in commercial, amenity or urban horticulture. Papers, including reviews, that give new insights into plant and crop growth, yield, quality and response to the environment, are welcome, including those arising from technological innovation and developments in crop genome sequencing and other biotechnological advances.
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