通过优化的化学处理,设计改进的脱细胞神经移植物。

Terry W. Hudson, Stephen Y Liu, C. Schmidt
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引用次数: 304

摘要

我们研究的长期目标是设计一种用于临床神经修复的无细胞神经移植物,并作为研究神经再生过程中神经-细胞外基质相互作用的模型系统。为了开发这种脱细胞神经移植模型,我们(1)检查了洗涤剂对周围神经组织的影响,(2)利用这些知识创建了一个没有细胞的神经移植物,具有保存良好的细胞外基质。使用组织化学和Western分析,确定每种洗涤剂对细胞和细胞外组织成分的影响。以Triton X-200、磺胺甜菜碱-16和磺胺甜菜碱-10为洗洁剂,建立了优化方案。这项研究代表了迄今为止清洁剂对周围神经组织形态和蛋白质组成的影响的最全面的检查。本文还提出了一种改进的化学脱细胞方案,该方案比现有的主要方案更能保留原生神经的内部结构。
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Engineering an improved acellular nerve graft via optimized chemical processing.
The long-term goal of our research is to engineer an acellular nerve graft for clinical nerve repair and for use as a model system with which to study nerve-extracellular matrix interactions during nerve regeneration. To develop this model acellular nerve graft we (1) examined the effects of detergents on peripheral nerve tissue, and (2) used that knowledge to create a nerve graft devoid of cells with a well-preserved extracellular matrix. Using histochemistry and Western analysis, the impact of each detergent on cellular and extracellular tissue components was determined. An optimized protocol was created with the detergents Triton X-200, sulfobetaine-16, and sulfobetaine-10. This study represents the most comprehensive examination to date of the effects of detergents on peripheral nerve tissue morphology and protein composition. Also presented is an improved chemical decellularization protocol that preserves the internal structure of native nerve more than the predominant current protocol.
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来源期刊
Tissue Engineering Part A
Tissue Engineering Part A CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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