软骨移植瘤和软骨细胞中连接蛋白43的表达与软骨分化的关系

Makio Nagano, H. Sakamaki, K. Yagami, S. Kakuta, M. Nagumo
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引用次数: 0

摘要

间隙连接与细胞生长和组织分化有关。然而,间隙连接与软骨形成或软骨分化的关系尚不清楚。我们成功地从人成软骨骨肉瘤在裸鼠身上建立了软骨移植瘤,并从移植肿瘤中获得了表达软骨细胞表型的细胞系(USAC)。利用这些移植肿瘤和USAC细胞,我们研究了间隙连接的表达与软骨形成或软骨分化之间的关系。通过连接蛋白43 (cx43)蛋白免疫染色观察间隙连接,该蛋白是间隙连接的固有成分。结果显示,cx43在肿瘤组织的软骨细胞和增生性细胞中均存在。在培养的细胞中,由cx43组成的间隙连接出现在USAC细胞中,被浓缩并向成熟的软骨细胞发展。高密度微质量培养USAC细胞发现,刺激软骨细胞分化的地塞米松和1a, 25 (OH)21)3可增强cx43的表达。这些结果表明,间隙连接的表达与软骨形成和软骨分化密切相关。
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Relationship between the Expression of Connexin 43 and Chondrogenic Differentiation in a Cartilaginous Transplanted Tumor and a Chondrocytic Cell Line
Gap junctions are associated with cell growth and differentiation i tissue organization. However, the association of gap junctions with chondrogenesis or chondrogenic differentiation isstill unclear. We succeeded in establishing a cartilaginous transplanted tumor in a nude mouse from a human chondroblastic osteosarcoma, nd a cell line (USAC) expressing chondrocytic phenotypes from the transplanted tumor. Using these transplanted tumor and USAC cells, we investigated the relationship between the expression of gap junctions and chondrogenesis or chondrogenic differentiation. Gap junctions are observed by immunostaining with connexin 43 (Cx 43) protein, which is an intrinsic component of gap junctions. The results revealed that Cx 43 in the tumor tissue was found both on chondrocytic and hypertrophic ells. In cultured cells, gap junctions composed of Cx 43 appearing as USAC cells were condensed and progressed toward mature chondrocytic ells. A high density micro-mass culture of USAC cells showed that dexamethasone and 1 a, 25 (OH)21)3, which stimulated chondrogenic differentiation, enhanced the expression of Cx 43. These results indicate that expression of gap junctions is closely linked to chondrogenesis and chondrogenic differentiation.
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