{"title":"咖啡因处理增强了低剂量γ辐照诱导的人白血病细胞染色单体型畸变,但对培养的人正常成纤维细胞没有影响","authors":"M. N. Jha, J. Bedford, S. Jha, K. Prasad","doi":"10.1504/IJLR.2011.047176","DOIUrl":null,"url":null,"abstract":"We investigated the effect of caffeine on low dose γ -radiationinduced chromosomal damage in human T-cell leukaemia cells (Jurkat T-cells) and two normal human fibroblast cell lines (AG1522 and GM2149). Low doses of gamma-radiation were found to increase the levels of chromatid gaps and breaks in a dose-dependent manner in both normal and cancer cells; however, cancer cells appeared to be more sensitive than the normal cells. Caffeine treatment before radiation exposure significantly increased the levels of chromatid gaps and breaks in Jurkat T-cells at all radiation doses, but it did not increase the level of these aberrations in normal cells. The mechanisms of this differential effect of caffeine in cancer cells and normal cells are unknown; however, G2-delay allows more time for rejoining of chromosome breakage to occur, then elimination of this delay by caffeine in tumour cells, not in normal cells might account for difference.","PeriodicalId":14141,"journal":{"name":"International Journal of Low Radiation","volume":"8 1","pages":"400"},"PeriodicalIF":0.0000,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1504/IJLR.2011.047176","citationCount":"1","resultStr":"{\"title\":\"Caffeine treatment enhances low dose γ-irradiation- induced chromatid-type aberrations in human leukaemia cells, but not in human normal fibroblast cells in culture\",\"authors\":\"M. N. Jha, J. Bedford, S. Jha, K. Prasad\",\"doi\":\"10.1504/IJLR.2011.047176\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We investigated the effect of caffeine on low dose γ -radiationinduced chromosomal damage in human T-cell leukaemia cells (Jurkat T-cells) and two normal human fibroblast cell lines (AG1522 and GM2149). Low doses of gamma-radiation were found to increase the levels of chromatid gaps and breaks in a dose-dependent manner in both normal and cancer cells; however, cancer cells appeared to be more sensitive than the normal cells. Caffeine treatment before radiation exposure significantly increased the levels of chromatid gaps and breaks in Jurkat T-cells at all radiation doses, but it did not increase the level of these aberrations in normal cells. The mechanisms of this differential effect of caffeine in cancer cells and normal cells are unknown; however, G2-delay allows more time for rejoining of chromosome breakage to occur, then elimination of this delay by caffeine in tumour cells, not in normal cells might account for difference.\",\"PeriodicalId\":14141,\"journal\":{\"name\":\"International Journal of Low Radiation\",\"volume\":\"8 1\",\"pages\":\"400\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1504/IJLR.2011.047176\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Low Radiation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1504/IJLR.2011.047176\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Low Radiation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1504/IJLR.2011.047176","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
Caffeine treatment enhances low dose γ-irradiation- induced chromatid-type aberrations in human leukaemia cells, but not in human normal fibroblast cells in culture
We investigated the effect of caffeine on low dose γ -radiationinduced chromosomal damage in human T-cell leukaemia cells (Jurkat T-cells) and two normal human fibroblast cell lines (AG1522 and GM2149). Low doses of gamma-radiation were found to increase the levels of chromatid gaps and breaks in a dose-dependent manner in both normal and cancer cells; however, cancer cells appeared to be more sensitive than the normal cells. Caffeine treatment before radiation exposure significantly increased the levels of chromatid gaps and breaks in Jurkat T-cells at all radiation doses, but it did not increase the level of these aberrations in normal cells. The mechanisms of this differential effect of caffeine in cancer cells and normal cells are unknown; however, G2-delay allows more time for rejoining of chromosome breakage to occur, then elimination of this delay by caffeine in tumour cells, not in normal cells might account for difference.
期刊介绍:
The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.