K. Nakashima, Miyuki Muraoka, S. Nakatsuji, S. Akiyama
{"title":"N -[4-(6-二甲氨基-2-苯并呋喃基)苯基]马来酰亚胺高效液相色谱法测定血清中d -青霉胺","authors":"K. Nakashima, Miyuki Muraoka, S. Nakatsuji, S. Akiyama","doi":"10.14921/JSCC1971B.17.2_51","DOIUrl":null,"url":null,"abstract":"A sensitive and simple high-performance liquid chromatographic determination method for D-penicillamine in serum was developed. The method is based on the fluorescence derivazation of D-penicillamine with N-[4-(6-dimethylamino-2-benzofuranyl)phenyl]maleimide and following fluorescence detection by high-performance liquid chromatography . A calibration graph for D-penicillamine was linear over the range from 200 fmol to 12 pmol , and the lower limit of detection at a signal-to-noise ratio of 2 was 50 fmol. Recovery rate of 2.9 ƒÊM of D-penicillamine was 91.8% (C.V.=4.2%, n=5). Time courses of serum levels of D-penicillamine after single oral administration of D-penicilamine in capsule to three women were examined. Serum levels reached maximum after 2 hr and then decreased gradually till 24 hr. This method will be useful for a monitoring of D-penicillamine in blood.","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Determination of D-Penicillamine in Serum by High-Performance Liquid Chromatography Using N -[4-(6-Dimethylamino-2-benzofuranyl) phenyl] maleimide\",\"authors\":\"K. Nakashima, Miyuki Muraoka, S. Nakatsuji, S. Akiyama\",\"doi\":\"10.14921/JSCC1971B.17.2_51\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A sensitive and simple high-performance liquid chromatographic determination method for D-penicillamine in serum was developed. The method is based on the fluorescence derivazation of D-penicillamine with N-[4-(6-dimethylamino-2-benzofuranyl)phenyl]maleimide and following fluorescence detection by high-performance liquid chromatography . A calibration graph for D-penicillamine was linear over the range from 200 fmol to 12 pmol , and the lower limit of detection at a signal-to-noise ratio of 2 was 50 fmol. Recovery rate of 2.9 ƒÊM of D-penicillamine was 91.8% (C.V.=4.2%, n=5). Time courses of serum levels of D-penicillamine after single oral administration of D-penicilamine in capsule to three women were examined. Serum levels reached maximum after 2 hr and then decreased gradually till 24 hr. This method will be useful for a monitoring of D-penicillamine in blood.\",\"PeriodicalId\":39360,\"journal\":{\"name\":\"Japanese Journal of Clinical Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese Journal of Clinical Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14921/JSCC1971B.17.2_51\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Clinical Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14921/JSCC1971B.17.2_51","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Determination of D-Penicillamine in Serum by High-Performance Liquid Chromatography Using N -[4-(6-Dimethylamino-2-benzofuranyl) phenyl] maleimide
A sensitive and simple high-performance liquid chromatographic determination method for D-penicillamine in serum was developed. The method is based on the fluorescence derivazation of D-penicillamine with N-[4-(6-dimethylamino-2-benzofuranyl)phenyl]maleimide and following fluorescence detection by high-performance liquid chromatography . A calibration graph for D-penicillamine was linear over the range from 200 fmol to 12 pmol , and the lower limit of detection at a signal-to-noise ratio of 2 was 50 fmol. Recovery rate of 2.9 ƒÊM of D-penicillamine was 91.8% (C.V.=4.2%, n=5). Time courses of serum levels of D-penicillamine after single oral administration of D-penicilamine in capsule to three women were examined. Serum levels reached maximum after 2 hr and then decreased gradually till 24 hr. This method will be useful for a monitoring of D-penicillamine in blood.
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