{"title":"酶反应器-电化学检测器高效液相色谱法测定多胺及其乙酰化衍生物","authors":"K. Hiramatsu, S. Kamei, K. Iwasaki","doi":"10.14921/JSCC1971B.18.4_191","DOIUrl":null,"url":null,"abstract":"A simple and sensitive high performance liquid chromatographic determination method for free polyamines and their acetylated derivatives was developed. Polyamines were separated on an octadecyl polymer column with isocratic elution. The separated polyamines were introduced into an enzyme reactor containing immobilized acylpolyamine amidohydrolase, amine oxidase (flavin-containing) and putrescine oxidase, in which they were deacetylated and oxidizld to generate hydrogen peroxide. The amount of hydrogen peroxide generated was then determined with an electrochemical detector. When a mixture of nine pure","PeriodicalId":39360,"journal":{"name":"Japanese Journal of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1989-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A New Determination Method for Polyamines and Their Acetylated Derivatives Involving High Performance Liquid Chromatography with an Enzyme Reactor and an Electrochemical Detector\",\"authors\":\"K. Hiramatsu, S. Kamei, K. Iwasaki\",\"doi\":\"10.14921/JSCC1971B.18.4_191\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A simple and sensitive high performance liquid chromatographic determination method for free polyamines and their acetylated derivatives was developed. Polyamines were separated on an octadecyl polymer column with isocratic elution. The separated polyamines were introduced into an enzyme reactor containing immobilized acylpolyamine amidohydrolase, amine oxidase (flavin-containing) and putrescine oxidase, in which they were deacetylated and oxidizld to generate hydrogen peroxide. The amount of hydrogen peroxide generated was then determined with an electrochemical detector. When a mixture of nine pure\",\"PeriodicalId\":39360,\"journal\":{\"name\":\"Japanese Journal of Clinical Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese Journal of Clinical Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14921/JSCC1971B.18.4_191\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Clinical Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14921/JSCC1971B.18.4_191","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
A New Determination Method for Polyamines and Their Acetylated Derivatives Involving High Performance Liquid Chromatography with an Enzyme Reactor and an Electrochemical Detector
A simple and sensitive high performance liquid chromatographic determination method for free polyamines and their acetylated derivatives was developed. Polyamines were separated on an octadecyl polymer column with isocratic elution. The separated polyamines were introduced into an enzyme reactor containing immobilized acylpolyamine amidohydrolase, amine oxidase (flavin-containing) and putrescine oxidase, in which they were deacetylated and oxidizld to generate hydrogen peroxide. The amount of hydrogen peroxide generated was then determined with an electrochemical detector. When a mixture of nine pure
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