{"title":"利用叶绿体DNA条形码分析探讨Parthenium杂草的生物型","authors":"T. Anjum, Rasheda Jabeen, S. Adkins, W. Akram","doi":"10.1590/s0100-83582020380100052","DOIUrl":null,"url":null,"abstract":"Background:Parthenium weed (Parthenium hysterophorus L.) is an invasive weed that has invaded vast regions of Pakistan in a relatively very short period of a decade or two, threatening the crop fields of the agrarian fed country. Parthenium hysterophorus L. is native of central South America and Gulf of Mexico, has now turned out to be a weed of global significance due to its alarming invasions and profuse spread in approximately all parts of the world. Its invasion is probably due to the contamination of its seeds in the imported grains from other countries of the world.Objective:During comprehensive sampling from Pakistan and Australia, it was observed that parthenium weed accessions exhibited several distinct morphological features present at different geographical regions. Therefore this study focuses on the use of plastid DNA barcodes (psbA-trnH) to evaluate the extent of variations in nucleotide sequences between the parthenium weed sampled accessions.Methods:The variability or genetic diversity was evaluated through sequencing of the amplified products and data was subjected to phylogenetic analysis in Molecular Evolutionary Genetic Analysis (MEGA; version 6.06) software.Results:In Maximum Likelihood tree, mainly two clades with three subdivisions are evident which showed increased heterogenity. The results of sequence based markers showed 12 haplotypes of P.hysterophorus populations (having two parsimony informative sites) with 10 indels and a few SNPs (single nucleotide polymorphisms).","PeriodicalId":20102,"journal":{"name":"Planta Daninha","volume":"1 1","pages":""},"PeriodicalIF":0.8000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring Parthenium weed biotypes by chloroplast DNA barcode analysis\",\"authors\":\"T. Anjum, Rasheda Jabeen, S. Adkins, W. Akram\",\"doi\":\"10.1590/s0100-83582020380100052\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background:Parthenium weed (Parthenium hysterophorus L.) is an invasive weed that has invaded vast regions of Pakistan in a relatively very short period of a decade or two, threatening the crop fields of the agrarian fed country. Parthenium hysterophorus L. is native of central South America and Gulf of Mexico, has now turned out to be a weed of global significance due to its alarming invasions and profuse spread in approximately all parts of the world. Its invasion is probably due to the contamination of its seeds in the imported grains from other countries of the world.Objective:During comprehensive sampling from Pakistan and Australia, it was observed that parthenium weed accessions exhibited several distinct morphological features present at different geographical regions. Therefore this study focuses on the use of plastid DNA barcodes (psbA-trnH) to evaluate the extent of variations in nucleotide sequences between the parthenium weed sampled accessions.Methods:The variability or genetic diversity was evaluated through sequencing of the amplified products and data was subjected to phylogenetic analysis in Molecular Evolutionary Genetic Analysis (MEGA; version 6.06) software.Results:In Maximum Likelihood tree, mainly two clades with three subdivisions are evident which showed increased heterogenity. The results of sequence based markers showed 12 haplotypes of P.hysterophorus populations (having two parsimony informative sites) with 10 indels and a few SNPs (single nucleotide polymorphisms).\",\"PeriodicalId\":20102,\"journal\":{\"name\":\"Planta Daninha\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2020-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Planta Daninha\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1590/s0100-83582020380100052\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Planta Daninha","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/s0100-83582020380100052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Exploring Parthenium weed biotypes by chloroplast DNA barcode analysis
Background:Parthenium weed (Parthenium hysterophorus L.) is an invasive weed that has invaded vast regions of Pakistan in a relatively very short period of a decade or two, threatening the crop fields of the agrarian fed country. Parthenium hysterophorus L. is native of central South America and Gulf of Mexico, has now turned out to be a weed of global significance due to its alarming invasions and profuse spread in approximately all parts of the world. Its invasion is probably due to the contamination of its seeds in the imported grains from other countries of the world.Objective:During comprehensive sampling from Pakistan and Australia, it was observed that parthenium weed accessions exhibited several distinct morphological features present at different geographical regions. Therefore this study focuses on the use of plastid DNA barcodes (psbA-trnH) to evaluate the extent of variations in nucleotide sequences between the parthenium weed sampled accessions.Methods:The variability or genetic diversity was evaluated through sequencing of the amplified products and data was subjected to phylogenetic analysis in Molecular Evolutionary Genetic Analysis (MEGA; version 6.06) software.Results:In Maximum Likelihood tree, mainly two clades with three subdivisions are evident which showed increased heterogenity. The results of sequence based markers showed 12 haplotypes of P.hysterophorus populations (having two parsimony informative sites) with 10 indels and a few SNPs (single nucleotide polymorphisms).
Planta DaninhaAgricultural and Biological Sciences-Plant Science
自引率
0.00%
发文量
0
审稿时长
16 weeks
期刊介绍:
Planta Daninha is a scientific journal published by the Brazilian Society of Weed Science (SBCPD - Sociedade Brasileira da Ciência das Plantas Daninhas). Papers submitted for publication must be sent through an electronic system, on http://www.scielo.br/pd. Works may be written in Portuguese, English, or Spanish, and will be accepted after being reviewed and approved by the Editorial Board. Only papers that have not been published or submitted for publication in other media will be accepted. Articles in Portuguese will be translated to English after being properly corrected and authorized by the authors. Planta Daninha has with goal to publish genuine technical-scientific papers and literature reviews from a critical perspective on Biology, weed management, and related topics.