大豆Enlist™系酶抗氧化防御系统及ALA-D酶活性

IF 1.2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Bragantia Pub Date : 2022-01-01 DOI:10.1590/1678-4499.20220010
Ani Carla Concato, W. A. Tamagno, C. Alves, Nathália Tafarel Sutorillo, A. P. Vanin, L. Vargas, R. Kaizer, L. Galon
{"title":"大豆Enlist™系酶抗氧化防御系统及ALA-D酶活性","authors":"Ani Carla Concato, W. A. Tamagno, C. Alves, Nathália Tafarel Sutorillo, A. P. Vanin, L. Vargas, R. Kaizer, L. Galon","doi":"10.1590/1678-4499.20220010","DOIUrl":null,"url":null,"abstract":": Enlist™ technology confers resistance to 2,4-D herbicides, glyphosate, and ammonium glufosinate for soybeans. However, the application of herbicides to plants can generate stress, even in resistant crops. Thus, here we evaluated whether the application of herbicides in transgenic soybean farms, resistant to these herbicides, adversely affects the activity of antioxidant enzymes and porphobilinogen formation by delta-aminolevulinate dehydratase (ALA-D) activity. At seven days after herbicide application, the aerial part of the plants was collected and used to determine the activity of antioxidant enzymes as catalase, ascorbate peroxidase, and guaiacol peroxidase, such as lipid peroxidation levels and the activity of the ALA-D enzyme. At seven days after herbicide application, the aerial part of plants was collect and used to determine the activity of antioxidant enzymes, such as catalase, ascorbate peroxidase and guaiacol peroxidase, as well as the levels of lipid peroxidation and the activity of the ALA-D enzyme. The activity of important antioxidant enzymes involved in the scavenging of reactive oxygen species (ROS) was increased in the soybean cultivar with the Enlist™ technology, while no severe damage to lipids was detected. However, the activity of ALA-D was inhibited, which could potentially impair the formation of porphobilinogen and decrease photosynthetic efficiency. Thus, these results indicate that herbicides can affect the activity of ROS-scavenging enzymes even in transgenic, herbicide-resistant plants.","PeriodicalId":9260,"journal":{"name":"Bragantia","volume":"1 1","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enzymatic antioxidant defense system and ALA-D enzyme activity in soybean Enlist™ line\",\"authors\":\"Ani Carla Concato, W. A. Tamagno, C. Alves, Nathália Tafarel Sutorillo, A. P. Vanin, L. Vargas, R. Kaizer, L. Galon\",\"doi\":\"10.1590/1678-4499.20220010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\": Enlist™ technology confers resistance to 2,4-D herbicides, glyphosate, and ammonium glufosinate for soybeans. However, the application of herbicides to plants can generate stress, even in resistant crops. Thus, here we evaluated whether the application of herbicides in transgenic soybean farms, resistant to these herbicides, adversely affects the activity of antioxidant enzymes and porphobilinogen formation by delta-aminolevulinate dehydratase (ALA-D) activity. At seven days after herbicide application, the aerial part of the plants was collected and used to determine the activity of antioxidant enzymes as catalase, ascorbate peroxidase, and guaiacol peroxidase, such as lipid peroxidation levels and the activity of the ALA-D enzyme. At seven days after herbicide application, the aerial part of plants was collect and used to determine the activity of antioxidant enzymes, such as catalase, ascorbate peroxidase and guaiacol peroxidase, as well as the levels of lipid peroxidation and the activity of the ALA-D enzyme. The activity of important antioxidant enzymes involved in the scavenging of reactive oxygen species (ROS) was increased in the soybean cultivar with the Enlist™ technology, while no severe damage to lipids was detected. However, the activity of ALA-D was inhibited, which could potentially impair the formation of porphobilinogen and decrease photosynthetic efficiency. Thus, these results indicate that herbicides can affect the activity of ROS-scavenging enzymes even in transgenic, herbicide-resistant plants.\",\"PeriodicalId\":9260,\"journal\":{\"name\":\"Bragantia\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bragantia\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1590/1678-4499.20220010\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bragantia","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1590/1678-4499.20220010","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

: Enlist™技术赋予大豆对2,4- d除草剂、草甘膦和草铵膦的抗性。然而,对植物施用除草剂会产生压力,即使是对抗性作物也是如此。因此,我们在这里评估了除草剂在转基因大豆农场的应用是否会对抗氧化酶的活性和三角洲氨基乙酰酸脱水酶(ALA-D)活性形成的卟绿胆色素原产生不利影响。在施用除草剂后7 d,收集植株地上部分,测定过氧化氢酶、抗坏血酸过氧化物酶和愈创木酚过氧化物酶等抗氧化酶的活性,如脂质过氧化水平和ALA-D酶的活性。在施用除草剂后7 d,收集植株地上部分,测定过氧化氢酶、抗坏血酸过氧化物酶和愈创木酚过氧化物酶等抗氧化酶的活性,以及脂质过氧化水平和ALA-D酶的活性。使用Enlist™技术的大豆品种中,参与清除活性氧(ROS)的重要抗氧化酶的活性增加,但未检测到对脂质的严重损害。然而,ALA-D活性受到抑制,这可能会影响卟胆色素原的形成,降低光合效率。因此,这些结果表明,除草剂可以影响ros清除酶的活性,甚至在转基因,抗除草剂的植物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Enzymatic antioxidant defense system and ALA-D enzyme activity in soybean Enlist™ line
: Enlist™ technology confers resistance to 2,4-D herbicides, glyphosate, and ammonium glufosinate for soybeans. However, the application of herbicides to plants can generate stress, even in resistant crops. Thus, here we evaluated whether the application of herbicides in transgenic soybean farms, resistant to these herbicides, adversely affects the activity of antioxidant enzymes and porphobilinogen formation by delta-aminolevulinate dehydratase (ALA-D) activity. At seven days after herbicide application, the aerial part of the plants was collected and used to determine the activity of antioxidant enzymes as catalase, ascorbate peroxidase, and guaiacol peroxidase, such as lipid peroxidation levels and the activity of the ALA-D enzyme. At seven days after herbicide application, the aerial part of plants was collect and used to determine the activity of antioxidant enzymes, such as catalase, ascorbate peroxidase and guaiacol peroxidase, as well as the levels of lipid peroxidation and the activity of the ALA-D enzyme. The activity of important antioxidant enzymes involved in the scavenging of reactive oxygen species (ROS) was increased in the soybean cultivar with the Enlist™ technology, while no severe damage to lipids was detected. However, the activity of ALA-D was inhibited, which could potentially impair the formation of porphobilinogen and decrease photosynthetic efficiency. Thus, these results indicate that herbicides can affect the activity of ROS-scavenging enzymes even in transgenic, herbicide-resistant plants.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Bragantia
Bragantia AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
2.40
自引率
8.30%
发文量
33
审稿时长
4 weeks
期刊介绍: Bragantia é uma revista de ciências agronômicas editada pelo Instituto Agronômico da Agência Paulista de Tecnologia dos Agronegócios, da Secretaria de Agricultura e Abastecimento do Estado de São Paulo, com o objetivo de publicar trabalhos científicos originais que contribuam para o desenvolvimento das ciências agronômicas. A revista é publicada desde 1941, tornando-se semestral em 1984, quadrimestral em 2001 e trimestral em 2005. É filiada à Associação Brasileira de Editores Científicos (ABEC).
期刊最新文献
Corn straw return effectively improves the stability and increases the carbon and nitrogen contents of waterstable aggregates in northeastern China black soil Spermine decreases ethylene and increases sugars and phenolic compounds in nasturtium flowers grown under drought and salt stress Genetic divergence for seed traits in Passiflora full-sib families under recurrent selection for resistance to the cowpea aphid-borne mosaic virus Physiological and agronomic response of soybean cultivars to soil compaction in the Brazilian Cerrado Training systems improved agronomic characteristics and quality of ‘Niagara Rosada’ table grapes
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1