伊拉姆市尿路感染中变形杆菌的表型及对抗生素的分子耐药性评价

A. Rostamzad, Khadijeh Fatahi, M. Nemati
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摘要

病原菌对抗生素的耐药性已成为一个世界性的问题,严重影响着传染病的治疗。本研究的目的是评估临床变形杆菌分离株的抗生素耐药性以及质粒转移耐药性的检测。材料与方法:收集尿路感染(UTI)患者的尿液样本250份,分别用血琼脂和麦康基琼脂培养。常规微生物及生化试验均诊断培养阳性。采用纸片扩散法进行药敏试验。采用琼脂稀释法测定最小抑菌浓度(MIC),并将质粒转化至无质粒的大肠杆菌ATCC 25922作为感受态细胞,测定质粒介导的抗生素耐药性。结果:200份标本中,女性标本采集120份(60%),男性标本分离80份(40%)。25种中诊断为变形杆菌(12.5%)。所有分离株对氨苄西林耐药(频率最高),只有16%的分离株对阿米卡星耐药(频率最低)。66.66%的变形杆菌分离株含有质粒。菌株对阿莫西林、氨苄西林的耐药率依次为:阿米卡星(88%)、庆大霉素(72%)、四环素(50%)、托布霉素(48%)、头孢他啶、头孢噻肟(32%)、环丙沙星(22%)。结论:抗生素的广泛使用导致细菌间通过r质粒转移产生或传播耐药性。
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The evaluation of phenotyping and molecular resistance to antibiotics in Proteus species isolated from urinary tract infections in Ilam city
Introduction: Resistance of pathogenic organisms to countenance antibiotics has become a worldwide problem with serious consequences on the treatment of infectious diseases. The aim of this study was to evaluate antibiotic resistance and also the detection of transferred antibiotic resistance by plasmid in clinical Proteus isolates. Materials and methods: A total of 250 urine samples were collected from patient suffered from urinary tract infection (UTI), and cultured on blood agar and MacConkey's agar. Positive cultures were diagnosed by routine microbiological and biochemical tests. Antibiotic susceptibility test was performed by disc diffusion method. The minimum inhibitory concentration (MIC) was evaluated by agar dilution method, and also antibiotic resistance mediated by plasmid was determined using transformation of plasmids to plasmid free Escherichia coli ATCC 25922 as competent cell. Results: Among 200 samples, 120 samples (60%) were collected from female and 80 samples (40%) were isolated from males. Out of 25 species (12.5%) were diagnosed as Proteus. Al isolates were resistant to ampicillin (maximum frequency), only 16% of isolates were resistance to amikacin (minimum resistance). Totally, 66.66% of Proteus isolates harbored plasmids. All plasmid containing P. mirabilis isolates were able to transferred resistance to amoxicillin, ampicillin, while rate of resistance to other antibiotics were as amikacin (88%), gentamycin (72%), tetracycline (50%), tobramycin (48%), ceftazidime, cefotaxime (32%) and ciprofloxacin (22%). Conclusion: Widespread use of antibiotics cause to spread or emerge antibiotic resistances among bacteria by R–plasmids transfer.
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