2020年4月至12月，莫斯科医护人员中抗严重急性呼吸系统综合征冠状病毒2型抗体的血清流行率

Q4 Medicine Infektsionnye Bolezni Pub Date : 2021-01-01 DOI:10.20953/1729-9225-2021-3-5-13

A. Popova, E.B. Yezhlova, A. A. Melnikova, E. E. Andreeva, S. Kombarova, A. V. Aleshkin, Yu.V. Kobzeva, E. N. Ignatova, M. Osadchaya, E.V. Nazarenko, L. Antipova, L. Novikova, S. Bochkareva, A. Basov, A. M. Zatevalov, E. I. Likhanskaya, T. Mizaeva, A. M. Vorobev, A. Galitskiy, S. D. Mitrokhin, A. S. Shkoda

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引用次数: 2

摘要

我们评估了为COVID-19患者提供住院护理的医护人员对严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)的体液免疫(特异性IgM和IgG的存在)。目标。检测和测量医护人员体内抗sars - cov -2抗体的水平，并确定其中有多少人接触过COVID-19患者，包括无症状或亚临床疾病的患者。材料和方法。采用酶联免疫吸附试验(ELISA)检测抗sars - cov -2抗体，IgG用“SARS-CoV-2-ELISA- Vector”试剂盒(国家病毒学与生物技术研究中心“Vector”)检测，IgM和IgG用“SARS-CoV-2-IgGELISA-BEST”和“SARS-CoV-2-IgМ-ELISA-BEST”试剂盒(Vector- best LLC)检测。样本是在莫斯科的几个卫生保健机构和盖布里切夫斯基莫斯科流行病学和微生物学研究所收集的。对来自74家卫生保健机构的24,373份血清标本进行了检测。所有的研究参与者都填写了特殊的问卷。结果。在这项研究中检测的24,373名医护人员中，有5,382人对SARS-CoV-2呈igg阳性(血清阳性率指数为22.1%)。高级医务人员血清阳性率为21.5%，护理专业人员血清阳性率为22.2%，医务助理/技术人员血清阳性率为22.8%。我们发现，SARS-CoV-2血清阳性个体的比例随着时间的推移而变化，从2020年4月的17.9%逐渐上升到2020年12月的37.6%。在这一年中，高级医务人员和护理专业人员中SARS-CoV-2血清阳性人群的比例翻了一番。无症状或亚临床感染者比例为13.8%。结论。本研究中使用的方法学方法使我们能够评估莫斯科医疗保健专业人员对SARS-CoV-2的体液免疫。本研究结果可为进一步完善卫生机构的防疫措施提供参考。关键词:血清阳性率，SARS-CoV-2，医护人员，酶联免疫吸附试验，IgG, IgM

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Seroprevalence of anti-SARS-CoV-2 antibodies among healthcare professionals in Moscow in April–December 2020

We evaluated humoral immunity (presence of specific IgM and IgG) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among healthcare professionals providing inpatient care for individuals with COVID-19. Objective. To detected and measure the level of anti-SARS-CoV-2 antibodies in healthcare professionals and to identify how many of them contacted with COVID-19 patients, including those who had asymptomatic or subclinical disease. Materials and methods. Anti-SARS-CoV-2 antibodies were detected using enzyme-linked immunosorbent assay (ELISA) with the ‘SARS-CoV-2-ELISA- Vector’ kit for IgG (State research Center of Virology and Biotechnology ‘Vector’) and ‘SARS-CoV-2-IgGELISA-BEST’ and ‘SARS-CoV-2-IgМ-ELISA-BEST’ kits for IgM and IgG (Vector-Best LLC). Samples were collected in several healthcare institutions of Moscow and in G.N.Gabrichevskiy Moscow Research Institute of Epidemiology and Microbiology. A total of 24,373 serum specimens from 74 healthcare institutions were tested. All study participants filled in special questionnaires. Results. Among 24,373 healthcare professionals tested in this study, 5,382 people were IgG-positive to SARS-CoV-2 (seroprevalence index 22.1%). The seropositivity rate was 21.5% in senior medical staff, 22.2% in nursing professionals, and 22.8% in medical assistants/technicians. We found that the proportion of SARS-CoV-2 seropositive individuals had changed over time, gradually increasing from 17.9% in April 2020 to 37.6% in December 2020. The proportion of SARS-CoV-2 seropositive people doubled among senior medical staff and nursing professionals during the year. The proportion of individuals with asymptomatic or subclinical COVID-19 was 13.8%. Conclusion. The methodological approaches used in this study allowed us to assess the humoral immunity to SARS-CoV-2 among healthcare professionals in Moscow. Our findings can be used for further improvement of anti-epidemic measures in healthcare institutions. Key words: seroprevalence, SARS-CoV-2, healthcare professionals, enzyme-linked immunosorbent assay, IgG, IgM

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来源期刊

Infektsionnye Bolezni Medicine-Infectious Diseases

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The journal publishes original research works, reviews of literature, lectures, methodological recommendations, clinical observations. Main topics: problems of etiology, pathogenesis, clinical manifestations of infectious diseases, new techniques and methods of their diagnosis, prevention and treatment; special attention is paid to the problems of antibacterial and antiviral therapy, the use of immunoglobulins and interferons, and also to intensive therapy of critical states. The journal is in the List of leading scientific journals and periodicals of the Supreme Attestation Committee, where the principal results of doctoral dissertations should be published.