Y. Shakiba, S. E. Rezatofighi, S. M. Seyyednejad, M. Ardakani
{"title":"毛藻水醇和水乙酸提取物对口蹄疫病毒复制的抑制作用","authors":"Y. Shakiba, S. E. Rezatofighi, S. M. Seyyednejad, M. Ardakani","doi":"10.22034/IJPS.2018.32052","DOIUrl":null,"url":null,"abstract":"Foot-and-mouth disease (FMD) is a major infectious disease of cloven-hoofed animals that is caused by the FMD virus (FMDV). This disease has significantly adverse economic impacts; therefore, rapid control measures are urgently. Traditional ranchers in Iran use Alhagi maurorum Medik. to treat FMD; therefore, we aimed to examine the antiviral activity of methanolic, ethanolic, and aqueous-acetic acid extracts of this plant against FMDV. The cytotoxicity of the extracts was assayed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) method and the antiviral activity of the extracts was evaluated by measuring the percentage of viable FMDV infected-cells via the MTT assay at different stages of the virus replication cycle. The results indicated that the plant extracts exhibit antiviral activity against FMDV at all stages of the experiment, although the most significant effects were observed in virucidal and pre-treatment assays. These findings represent the anti-FMDV activities of A. maurorum extracts at several stages of the virus replication cycle; therefore, it could be considered for the potential development of anti-FMDV therapeutics. This creates the basis for further analyses of the molecular mechanisms of the antiviral activity of this herbal plant.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"14 1","pages":"85-96"},"PeriodicalIF":0.0000,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Inhibition of Foot-and-Mouth Disease Virus Replication by Hydro-alcoholic and Aqueous-Acetic Acid Extracts of Alhagi maurorum\",\"authors\":\"Y. Shakiba, S. E. Rezatofighi, S. M. Seyyednejad, M. Ardakani\",\"doi\":\"10.22034/IJPS.2018.32052\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Foot-and-mouth disease (FMD) is a major infectious disease of cloven-hoofed animals that is caused by the FMD virus (FMDV). This disease has significantly adverse economic impacts; therefore, rapid control measures are urgently. Traditional ranchers in Iran use Alhagi maurorum Medik. to treat FMD; therefore, we aimed to examine the antiviral activity of methanolic, ethanolic, and aqueous-acetic acid extracts of this plant against FMDV. The cytotoxicity of the extracts was assayed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) method and the antiviral activity of the extracts was evaluated by measuring the percentage of viable FMDV infected-cells via the MTT assay at different stages of the virus replication cycle. The results indicated that the plant extracts exhibit antiviral activity against FMDV at all stages of the experiment, although the most significant effects were observed in virucidal and pre-treatment assays. These findings represent the anti-FMDV activities of A. maurorum extracts at several stages of the virus replication cycle; therefore, it could be considered for the potential development of anti-FMDV therapeutics. This creates the basis for further analyses of the molecular mechanisms of the antiviral activity of this herbal plant.\",\"PeriodicalId\":14582,\"journal\":{\"name\":\"Iranian Journal of Pharmaceutical Sciences\",\"volume\":\"14 1\",\"pages\":\"85-96\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Pharmaceutical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22034/IJPS.2018.32052\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22034/IJPS.2018.32052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
Inhibition of Foot-and-Mouth Disease Virus Replication by Hydro-alcoholic and Aqueous-Acetic Acid Extracts of Alhagi maurorum
Foot-and-mouth disease (FMD) is a major infectious disease of cloven-hoofed animals that is caused by the FMD virus (FMDV). This disease has significantly adverse economic impacts; therefore, rapid control measures are urgently. Traditional ranchers in Iran use Alhagi maurorum Medik. to treat FMD; therefore, we aimed to examine the antiviral activity of methanolic, ethanolic, and aqueous-acetic acid extracts of this plant against FMDV. The cytotoxicity of the extracts was assayed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) method and the antiviral activity of the extracts was evaluated by measuring the percentage of viable FMDV infected-cells via the MTT assay at different stages of the virus replication cycle. The results indicated that the plant extracts exhibit antiviral activity against FMDV at all stages of the experiment, although the most significant effects were observed in virucidal and pre-treatment assays. These findings represent the anti-FMDV activities of A. maurorum extracts at several stages of the virus replication cycle; therefore, it could be considered for the potential development of anti-FMDV therapeutics. This creates the basis for further analyses of the molecular mechanisms of the antiviral activity of this herbal plant.
期刊介绍:
Iranian Journal of Pharmaceutical Sciences (IJPS) is an open access, internationally peer-reviewed journal that seeks to publish research articles in different pharmaceutical sciences subdivisions: pharmacology and toxicology, nanotechnology, pharmaceutics, natural products, biotechnology, pharmaceutical chemistry, clinical pharmacy and other pharmacy related topics. Each issue of the journal contents 16 outstanding research articles in area of pharmaceutical sciences plus an editorial written by the IJPS editors on one of the most up to date advances topics in pharmacy. All articles published by IJPS would be permanently accessible online freely without any subscription charges. Authors of the published articles have granted the right to use and disseminate their article to third parties.