四种植物提取物对牛斑库伯氏线虫的杀卵活性

IF 0.2 4区 农林科学 Q2 Veterinary Veterinaria Mexico Pub Date : 2016-06-27 DOI:10.21753/VMOA.3.2.365
E. V. S. Fernex, M. A. Diaz, P. M. Gives, B. V. L. Mora, A. Zamilpa, M. G. Cortazar
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引用次数: 14

摘要

引用本文:von Son de Fernex E, Alonso Diaz MA, Mendoza de Gives P, Valles de la Mora B, Zamilpa A, Gonzalez Cortasar M.四种植物提取物对牛斑库布线虫的杀卵活性研究。墨西哥兽医局。2016; 3(2)。Doi: 10.21753/vmoa.3.2.365。具有生物活性的植物可能是控制放牧牛斑枯蝇的替代选择。本研究的目的是(1)评价4种植物提取物对刺毛虫的杀卵活性,(2)确定多酚在植物驱虫活性中的作用,(3)在寻找杀卵活性时评价最佳的植物提取工艺。采用水浸、丙酮:水浸、丙酮:水浸三种不同的浸提方法,对白头翁、白头翁、白头翁和白头翁的杀卵活性进行了评价。每株植物提取物分别为0.6、1.2、2.4、4.8和9.6 mg mL -1。用聚乙二醇(PEG)评价了多酚的作用。12种植物提取物抑制卵孵化呈剂量依赖性。G. sepium -AC和L. leucocephala -AQ的最佳lc50值分别为1.03±0.17和7.90±1.19 mg mL -1。不同提取工艺间AH活性差异有统计学意义(P < 0.05)。在最高浓度下,L. leucocephala -AQ对点状棘球蚴的孵化抑制率达50%以上。黄皮草-AC提取物能完全抑制卵的孵化。聚乙二醇的加入揭示了多酚在大多数植物提取物生物活性中的作用;然而,对于G. sepium -AC,多酚不是主要的生物活性物质。综上所述,丙酮-水萃取法是获得除卵活性和较高产率的最佳提取工艺。从抑制率来看,应考虑将白头L.和粗皮G.作为降低牧场幼虫密度的一种手段。表1。生物活性提取物(mg mL -1)孵育48 h后,所需的致死浓度可抑制50%的斑点库伯卵孵化(lc50)。
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Ovicidal activity of extracts from four plant species against the cattle nematode Cooperia punctata
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: von Son de Fernex E, Alonso Diaz MA, Mendoza de Gives P, Valles de la Mora B, Zamilpa A, Gonzalez Cortasar M. Ovicidal activity of extracts from four plant species against the cattle nematode Cooperia punctata . Veterinaria Mexico OA. 2016;3(2). doi: 10.21753/vmoa.3.2.365 . Bioactive plants might represent an alternative for Cooperia punctata control in grazing cattle. The objectives of this study were (1) to assess the ovicidal activity of extracts from 4 plant species against C. punctata , (2) to determine the role of the polyphenols in the plants’ anthelmintic (AH) activity, and (3) to evaluate the best plant extraction procedure when searching for ovicidal activity. The egg hatch assay was used with different extraction procedures, aqueous (AQ), acetone:water (AW) and acetonic (AC), to evaluate the ovicidal activity of Leucaena leucocephala , Gliricidia sepium , Guazuma ulmifolia and Cratylia argentea . Eggs of C. punctata were exposed in quadruplicate to 0.6, 1.2, 2.4, 4.8 and 9.6 mg mL -1 of each plant extract. The roles of the polyphenols were assessed using polyethylene glycol (PEG). The 12 plant extracts inhibited egg hatching in a dose-dependent manner. Best-fit LC 50 values were 1.03 ± 0.17 and 7.90 ± 1.19 mg mL -1 for G. sepium -AC and L. leucocephala -AQ, respectively. Differences in AH activity were found among the extraction procedures (P < 0.05). At the highest concentration, L. leucocephala -AQ inhibited more than 50% of C. punctata hatching . The G. sepium -AC extract fully inhibited egg hatching. The addition of polyethylene glycol revealed the role of the polyphenols in the bioactivity of most plant extracts; however, for G. sepium -AC, the polyphenols were not the main bioactive compounds. Overall, acetone:water extraction represented the best extraction procedure to obtain both ovicidal activity and higher yield. The inhibition rates suggested that L. leucocephala and G. sepium should be evaluated as a means of reducing larval density in pastures. Table 1. Lethal concentrations required to inhibit 50% of Cooperia punctata egg hatching (LC 50 ), after a 48-h incubation period with bioactive extracts (mg mL -1 ).
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Veterinaria Mexico
Veterinaria Mexico VETERINARY SCIENCES-
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期刊介绍: Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).
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