Cristina Ibarra Zazueta, B. A. Reynoso, R. Castro, E. G. P. Resendiz, E. D. Aparicio
{"title":"假结核棒状杆菌aroA突变体在细胞和小鼠模型中的评价","authors":"Cristina Ibarra Zazueta, B. A. Reynoso, R. Castro, E. G. P. Resendiz, E. D. Aparicio","doi":"10.21753/VMOA.3.4.366","DOIUrl":null,"url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Ibarra Zazueta C, Arellano Reynoso B, Hernandez Castro R, Palomares Resendiz EG, Diaz Aparicio E. Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models. Veterinaria Mexico OA. 2016;3(4). doi: 10.21753/vmoa.3.4.366 Caseous lymphadenitis of small ruminants causes economic losses worldwide. To date, no effective vaccine has been developed against the causative agent of this disease, Corynebacterium pseudotuberculosis . The objective of the present work was to evaluate an aroA mutant gene strain of C. pseudotuberculosis in cellular and murine models, for attenuation and the ability to stimulate an immune response. The intracellular survival of the aroA mutant strain and the wild type strain (WT) of C. pseudotuberculosis was evaluated in J774A.1 murine macrophages using a multiplicity of infection (MOI) of 1:1 with the following infection times: 30 min, and 1, 2, 4, 8, 12, and 24 h. The largest difference in the intracellular survival of the mutant was observed 30 min post-infection. After subcutaneous skin vaccination, the subcutaneous lesion progression observed on the 14 th day was more severe in those animals that were vaccinated with the WT strain. An analysis of the residual virulence in the murine model did not reveal any bacteria in mice vaccinated with the aroA strain on day 28 post-vaccination. Mice vaccinated with the mutant showed 50 % protection against the intraperitoneal challenge, exceeding that of the control group (41.67 %). We conclude that the virulence of the aroA mutant was significantly attenuated in both cellular and murine models according to the residual virulence detected in mice. However, vaccination with the mutant failed to confer at least 80 % protection, which is desirable for an immunogen. Hence, this study contributes to the knowledge of the immune response against Corynebacterium pseudotuberculosis .","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":"3 1","pages":"26-42"},"PeriodicalIF":0.2000,"publicationDate":"2016-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.3.4.366","citationCount":"1","resultStr":"{\"title\":\"Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models\",\"authors\":\"Cristina Ibarra Zazueta, B. A. Reynoso, R. Castro, E. G. P. Resendiz, E. D. Aparicio\",\"doi\":\"10.21753/VMOA.3.4.366\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Ibarra Zazueta C, Arellano Reynoso B, Hernandez Castro R, Palomares Resendiz EG, Diaz Aparicio E. Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models. Veterinaria Mexico OA. 2016;3(4). doi: 10.21753/vmoa.3.4.366 Caseous lymphadenitis of small ruminants causes economic losses worldwide. To date, no effective vaccine has been developed against the causative agent of this disease, Corynebacterium pseudotuberculosis . The objective of the present work was to evaluate an aroA mutant gene strain of C. pseudotuberculosis in cellular and murine models, for attenuation and the ability to stimulate an immune response. The intracellular survival of the aroA mutant strain and the wild type strain (WT) of C. pseudotuberculosis was evaluated in J774A.1 murine macrophages using a multiplicity of infection (MOI) of 1:1 with the following infection times: 30 min, and 1, 2, 4, 8, 12, and 24 h. The largest difference in the intracellular survival of the mutant was observed 30 min post-infection. After subcutaneous skin vaccination, the subcutaneous lesion progression observed on the 14 th day was more severe in those animals that were vaccinated with the WT strain. An analysis of the residual virulence in the murine model did not reveal any bacteria in mice vaccinated with the aroA strain on day 28 post-vaccination. Mice vaccinated with the mutant showed 50 % protection against the intraperitoneal challenge, exceeding that of the control group (41.67 %). We conclude that the virulence of the aroA mutant was significantly attenuated in both cellular and murine models according to the residual virulence detected in mice. However, vaccination with the mutant failed to confer at least 80 % protection, which is desirable for an immunogen. 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Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Ibarra Zazueta C, Arellano Reynoso B, Hernandez Castro R, Palomares Resendiz EG, Diaz Aparicio E. Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models. Veterinaria Mexico OA. 2016;3(4). doi: 10.21753/vmoa.3.4.366 Caseous lymphadenitis of small ruminants causes economic losses worldwide. To date, no effective vaccine has been developed against the causative agent of this disease, Corynebacterium pseudotuberculosis . The objective of the present work was to evaluate an aroA mutant gene strain of C. pseudotuberculosis in cellular and murine models, for attenuation and the ability to stimulate an immune response. The intracellular survival of the aroA mutant strain and the wild type strain (WT) of C. pseudotuberculosis was evaluated in J774A.1 murine macrophages using a multiplicity of infection (MOI) of 1:1 with the following infection times: 30 min, and 1, 2, 4, 8, 12, and 24 h. The largest difference in the intracellular survival of the mutant was observed 30 min post-infection. After subcutaneous skin vaccination, the subcutaneous lesion progression observed on the 14 th day was more severe in those animals that were vaccinated with the WT strain. An analysis of the residual virulence in the murine model did not reveal any bacteria in mice vaccinated with the aroA strain on day 28 post-vaccination. Mice vaccinated with the mutant showed 50 % protection against the intraperitoneal challenge, exceeding that of the control group (41.67 %). We conclude that the virulence of the aroA mutant was significantly attenuated in both cellular and murine models according to the residual virulence detected in mice. However, vaccination with the mutant failed to confer at least 80 % protection, which is desirable for an immunogen. Hence, this study contributes to the knowledge of the immune response against Corynebacterium pseudotuberculosis .
期刊介绍:
Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges.
This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).