微RNA表达与小儿乳糜泻临床表现的关系

4区 农林科学 Q3 Agricultural and Biological Sciences Genetika-Belgrade Pub Date : 2023-01-01 DOI:10.2298/gensr2301277d
G. Dogan, S. Boyacıoğlu, M. Caliskan, E. Kasap, S. Ayhan, E. Kasırga
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引用次数: 0

摘要

有必要确定儿童乳糜泻(pCD)中免疫系统功能与miRNA表达之间的关系。我们的目的是根据临床和病理结果描述mirna在土耳其pCD患者中的表达谱。本研究对33例pCD患者和33例活检结果正常的儿童对照组进行了研究。筛选出pCD患者HLA基因最常见的4个突变位点(DQA1*05、DQB1*02、DQA1*03、DQB1*03:0.2)。石蜡包埋活检组织样本用于miRNA分离,然后进行cDNA合成。采用qRT-PCR阵列法检测各组mirna的表达情况。与对照组相比,pCD患者中检测到hsa-miR-194-5p基因显著低表达。与对照组相比,hsa-miR-194-5p基因在贫血或身材矮小的pCD患者中显着低表达。hsa-miR-29b-3p、hsa-miR-30e-5p、hsa-miR-146a-5p基因在便秘型乳糜泻患者中显著过表达。在Marsh2和Marsh3a组中检测到hsa-miR146a-5p基因显著过表达。hsa-miR-29b-3p、hsa-miR-30e-5p、hsa-let-7a-5p、hsa-miR-27a-3p、hsa-miR141-3p、hsa-miR143-3p和hsa-miR-146a-5p miRNA基因在Marsh3b组中显著过表达。此外,与对照组相比,Marsh3c组的hsa-miR-194-5p和hsa-miR-26a-5p基因明显低表达。这些结果提示miRNA表达可能在pCD的发病机制中发挥作用。我们认为,目前的结果提供了有价值的推断,可能有助于了解pCD的遗传边界,这可能会进一步支持对其他mirna的后续研究。
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Association of micro RNA expressions with pediatric celiac clinical findings
There is a need to determine the relationship between the function of the immune system and miRNA expression in pediatric celiac disease (pCD). We aimed to describe the expression profiles of miRNAs in Turkish pCD patients based on the clinical and pathological findings. This study was conducted on 33 pCD patients and 33 pediatric control subjects with normal biopsy results. Four most common mutations (DQA1*05, DQB1*02, DQA1*03, DQB1*03:0.2) on HLA gene in pCD were screened. Paraffin-embedded biopsy tissue samples were used in miRNA isolations followed by cDNA synthesis. Expression of miRNAs were evaluated in the groups with qRT-PCR array-method. Significant underexpression of hsa-miR-194-5p gene was detected in pCD patients compared to the control group. The hsa-miR-194-5p gene was significantly underexpressed in anemic or short stature pCD patients compared to the control. The genes of hsa-miR-29b-3p, hsa-miR-30e-5p, and hsa-miR-146a-5p were significantly overexpressed in the patients with constipated celiac patients. Significant overexpression of hsa-miR146a-5p gene was detected in the Marsh2 and Marsh3a groups. The hsa-miR-29b-3p, hsa-miR-30e-5p, hsa-let-7a-5p, hsa-miR-27a-3p, hsa-miR141-3p, hsa-miR143-3p, and hsa-miR-146a-5p miRNA genes were significantly overexpressed in the Marsh3b group. Also, the hsa-miR-194-5p and hsa-miR-26a-5p genes were significantly underexpressed in the comparison of Marsh3c group to the control. These results suggest that miRNA expressions are likely to play a role in the pathogenesis of pCD. It is believed that the current results present valuable inferences that may help understand the genetic boundaries on pCD, which might be further supported by follow up studies on other miRNAs.
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来源期刊
Genetika-Belgrade
Genetika-Belgrade AGRONOMY-GENETICS & HEREDITY
CiteScore
1.80
自引率
0.00%
发文量
1
审稿时长
6-12 weeks
期刊介绍: The GENETIKA is dedicated to genetic studies of all organisms including genetics of microorganisms, plant genetics, animal genetics, human genetics, molecular genetics, genomics, functional genomics, plant and animal breeding, population and evolutionary genetics, mutagenesis and genotoxicology and biotechnology.
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