GENE MAPPING OF PHOSPHOGLYCEROL PHOSPHOLIPASE C HOMEOLOGS TO SINGLE CHROMOSOMES IN WHEAT (Triticum aestivum L.)

Phospholipasase Cs (PLCs) has been recognized as important enzymes for their roles in regulation, signal transduction and membrane composition. Two full length wheat PLCs cDNAs, Ta-PG-PLC2-1 and Ta-PG-PLC2-2 from wheat, Triticum aestivum L., were sequenced and mapped to wheat chromosome arms using PCR amplification in diploid wheat ancestors, Triticum urartu, Aegilops speltoides, Aegilops tauschii and cytogenetic stocks of Triticum aestivum and found to have very high sequence similarity and one, PG-PLC2-1, was found to contain a frame shift mutation 29 nt downstream of the first ATG start codon. This sequence was used to survey the prevalence of the mutation in twenty one wheat cultivars and was found to be present in all wheat cultivars tested and in the ancestral diploid species, Triticum urartu.