EFFECT OF EFFLUENT ON BACTERIA COMPARING WITH TAP WATER

Water is important for all the living things but nowadays, it has been polluted with inorganic contaminants which are discharged from industries. This study was carried out to evaluate the potential of Rhizobium leguminosarum, Azotobacter chrocoocum, Serratia marcescns and Bacillus circulans to survive in effluent obtained from Alexandria and Sadat City and the ability of these bacteria to remove metals from effluent. Samples of effluent were assessed for various physicochemical parameters like pH, Electrical conductivity (EC), concentration of salts, osmotic pressure, biological oxygen demand (BOD) (35 and 65), chemical oxygen demand (COD) (94 and 135) in Alexandria and Sadat City, respectively. The pH was 7.58 and 7.78, respectively compared with tap water (control) pH from Sadat City and Alexandria 7.4-7.2, respectively. It was found that different the concentrations of media containing effluent obtained from Alexandria and Sadat City having negative effect on bacterial growth and the more effective of Sadat City effluent, except Bacillus circulans had the same trend. The examined bacteria were capable of removing metals from different sources of effluent (Alexandria and Sadat City), Cu++ ions was highest removed by Rhizobium leguminosarum (44% and 39%), Azotobacter chrocoocum (42% and 50%), Serratia marcescns (50% and 50%) and Bacillus circulans (36% and 46%, respectively). The SDS- PAGE profiles of the extracted protein showed change in gene expression for protein profiles comparing with control. The incubation these bacteria in effluent increase superoxide dismutase (SOD) isozyme activity.