大麦耐水基因型的分子和田间分析

M. E. El-denary, E. E. EL-Shawy
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引用次数: 8

摘要

在田间和室内研究了3种大麦(Hordeum vulgare L.)基因型的水分胁迫效应。不同生育期对水分胁迫的基因型反应不同。室内试验表明,发芽率、茎长、根长和总干物质是敏感型和耐型之间最有效的性状。吉萨126和加州万豪在不同胁迫水平下均表现出较强的耐受性和稳定性,而敏感品种吉萨129的发芽率、茎长和总干物质均急剧下降。吉萨126在胁迫条件和良好灌溉条件下的产量和产量组成值最高,其次是加州万豪。吉萨129在压力条件下排名最后。结果表明,HVB23D SSR引物和LEA特异引物产生的特异条带在所研究的耐旱大麦基因型(Giza 126和California Marriott)中出现,而在敏感基因型Giza 129中不存在。
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MOLECULAR AND FIELD ANALYSIS OF SOME BARLEY GENO-TYPES FOR WATER STRESS TOLERANCE
Water stress effects on three barley (Hordeum vulgare L.) genotypes in field and lab were studied. The genotypic responses varied among growth stages according to water stress. Lab experiments showed that germination percentage, shoot length, root length and total dry mater were the most effective traits between sensitive and tolerant genotypes. Giza 126 and California Marriott were tolerant and stable under different stress levels, while the sensitive variety Giza 129 showed sharp decrease in germination percentage, shoot length and total dry mater. Giza 126 recorded the highest yield and yield component values under stress condition as well as in well-irrigated condition, followed by California Marriott under the stress condition. Giza 129 ranked last under stress condition. Our results showed that the HVB23D SSR primer and the specific primer LEA generated specific bands occurred in the studied drought tolerant barley genotypes (Giza 126 and California Marriott), while they were absent in the sensitive genotype Giza 129.
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