鳞状葡萄孢菌——波斯尼亚和黑塞哥维那洋葱叶枯病的病原体

B. Tanović, Milan Koščica, J. Hrustić, M. Mihajlović, V. Trkulja, G. Delibašić
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引用次数: 4

摘要

在过去几十年里,在波斯尼亚和黑塞哥维那集中种植洋葱的几个地方,在洋葱叶子上观察到坏死斑点、病变和枯萎症状。症状类型表明可能感染了鳞状葡萄孢菌,这是一种广泛存在的洋葱病原体。由于叶斑和坏死病变的症状也可能由其他一些生物和非生物因素引起,我们的研究重点是确定观察到的症状的病因。采用标准的植物病理学程序从病变组织中分离出病原体,并根据病原学和形态学特征进行鉴定。通过核糖体DNA (rDNA)内部转录间隔区(ITS)的扩增和测序证实了鉴定。研究了温度和培养基对分离菌株菌丝生长速率的影响。
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Botrytis squamosa - the causal agent of onion leaf blight in Bosnia and Herzegovina
Over the past several decades, necrotic spots, lesions and blight symptoms have been observed on onion leaves in several locations in Bosnia and Herzegovina, where the crop is grown intensively. The type of symptoms indicated a possible infection with Botrytis squamosa, a widespread pathogen of onion. As symptoms of leaf spots and necrotic lesions can also be caused by some other biotic and abiotic factors, our research focused on identifying the causal agent of the observed symptoms. The pathogen was isolated from diseased tissue using standard phytopathological procedure and identified based on pathogenic and morphological features. Identification was confirmed by amplification and sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA). The influence of temperature and growth medium on mycelial growth rate of the isolates was also studied.
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审稿时长
4 weeks
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