植物精油对辣椒黄萎病病原大丽花黄萎病菌的抗真菌活性

J. Lukovic, B. Todorovic, S. Milijasevic-Marcic, E. Rekanović, M. Kostić, Rada Djurovic-Pejcev, I. Potocnik
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引用次数: 3

摘要

基于植物油的生物杀菌剂与化学杀菌剂相比具有一些优势,特别是考虑到它们对环境的有害影响。采用大量稀释熏蒸法,对德国和阿尔巴尼亚产的22种精油对辣椒黄萎病病原菌大丽花黄萎病菌的抑菌活性进行了研究。在气相中得到的油的浓度分别为:0.02、0.04、0.08、0.16和0.32 μ l ml-1,并确定了最低抑菌和杀真菌浓度。两份来自德国和阿尔巴尼亚的薄荷油(Mentha piperita l .)在0.02 μ l ml-1的空气中表现出最强的活性,其次是桉树(eucalyptus globulus Labilardie)、黑松(Pinus nigra l .)和杜松(Juniperus oxycedrus l .)植物精油,均对病原菌具有致死性。薄荷、桉树、黑松、薰衣草(Lavandula angustifolia Mill.)、鼠尾草(Salvia officinalis L.)和迷迭香(Rosmarinus officinalis L.) 9种精油对大丽花黄萎病菌的生长有抑制作用,薄荷、桉树、黑松和黑松2种精油对大丽花黄萎病菌有杀真菌作用。结果表明,薄荷精油、桉树精油、黑松精油和栀子精油具有进一步抗黄萎病体内实验的潜力。
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Antifungal activity of plant essential oils against Verticillium dahliae Klebahn, the causal agent of verticillium wilt of pepper
Biofungicides based on plant oils have some advantages compared to chemical fungicides, especially considering their harmful effect on the environment. Twenty-two essential oils from Germany and Albania were assayed for inhibitory and fungicidal activity against Verticillium dahliae Klebahn, the causal agent of Verticillium wilt of pepper, using the macrodilution fumigant method. The concentrations of oils obtained in the vapour phase were: 0.02, 0.04, 0.08, 0.16 and 0.32 ?l ml-1 with determined minimum inhibitory and fungicidal concentrations. The strongest activity was shown by two samples of mint oil (Mentha piperita L.) at 0.02 ?l ml-1 of air, both from Germany and Albania, followed by plant essential oils of eucalyptus (Eucalyptus globulus Labilardie), black pine (Pinus nigra L.) and cade (Juniperus oxycedrus L.), and all of them were lethal to the pathogen. Nine oils: two samples of mint, cade, eucalyptus, black pine, lavender (Lavandula angustifolia Mill.), sage (Salvia officinalis L.) and rosemary (Rosmarinus officinalis L.) inhibited the growth of Verticillium dahliae, while five oils (two samples of mint, eucalyptus, black pine and cade) showed fungicidal effects on the pathogen. These results showed that mint, eucalyptus, black pine and cade essential oils have a potential for further in vivo experiments against Verticillium dahliae.
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