塞尔维亚油菜黄单胞菌的等位基因分析。白菜中的Campestris分离株

T. Popović, A. Jelušić, P. Mitrović, R. Iličić, S. Markovic
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引用次数: 1

摘要

油菜黄单胞菌。campestris (Xcc)是白菜黑腐病的病原菌,是影响白菜正常生长的重要细菌之一,导致白菜头重和品质损失,从而大大降低白菜的市场价值。这种病原体具有遗传多样性,这从全世界11个种族和30多种等位基因组合中可以明显看出。因此,本研究旨在确定2014年获得的塞尔维亚白菜Xcc菌株的等位基因谱。选择3株Xcc菌株进行分析,首先用PCR扩增4个内控基因P-XdnaK、fyuA、gyrB和rpoD进行测序,最后用测序结果确定等位基因谱。通过与来自不同寄主和地区的33株Xcc菌株进行比较,确定了等位基因谱。利用pubMLST的非冗余数据库(NRDB)确定等位基因图谱,利用Phyloviz软件构建最小生成树。所有塞尔维亚Xcc白菜品系的P-X-dnaK、fyuA、gyrB和rpoD基因等位基因谱分别为1、3、1和1。该基因型被确定为序列2型(ST2),它与葡萄牙甘蓝Xcc菌株CPBF 213一致,该菌株源自甘蓝变种。未检测到序列类型(ST)与主机之间的连接。
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Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage
Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which affect proper cabbage growth, leading to head weight and quality losses and thereby drastically reducing its marketing value. The pathogen is genetically diverse, which is evident from the presence of eleven races worldwide and more than thirty combinations of allelic profiles. Therefore, this study aimed to determine the allelic profiles of Serbian cabbage Xcc strains obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was first amplified using polymerase chain reaction (PCR) with four housekeeping genes - P-XdnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were finally used to determine allelic profiles. Allelic profiles were determined by comparison with 33 Xcc strains obtained from different hosts and regions, whose allelic profiles had been determined previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profile determination and Phyloviz software for constructing a minimum spanning tree. The obtained allelic profile of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and rpoD genes, respectively. This profile is assigned as sequence type 2 (ST2) and it coincides with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No connection between sequence type (ST) and the host was detected.
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