塞尔维亚小麦条纹花叶病毒的发生及分子特征

A. Vučurović, I. Stanković, K. Zečević, Branka Petrović, G. Delibašić, B. Krstic
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引用次数: 0

摘要

小麦条纹花叶病毒(WSMV)以小麦曲螨为媒介,在全球范围内广泛传播,严重威胁着小麦生产。自20世纪60年代在塞尔维亚首次出现以来,WSMV的存在一直没有得到监测。2019年,我们收集了来自塞尔维亚8个地点的5个小麦品种的62份样本,并使用单个或多重RT-PCR检测了9种常见小麦病毒的存在:WSMV、大麦黄矮病毒- pav、-MAV、-SGV和-RMV、谷物黄矮病毒- rpv、小麦纺轴条纹病毒、小麦花叶病毒和土壤传播的小麦花叶病毒。在5个不同地点的7种小麦作物中,58.1%的样品中检测到WSMV。物种特异性引物未能检测到其他8种测试病毒的存在。为了进一步证实WSMV的存在,利用覆盖外壳蛋白(CP)基因的WS8166F/WS8909R引物进行RT-PCR扩增和测序。选取菌株98 ~ 19、99 ~ 19、102 ~ 19和120 ~ 19的扩增产物进行测序并存入GenBank(分别为MT461299、MT461300、MT461301和MT461302)。塞尔维亚WSMV分离株具有非常高的核苷酸同源性(98.16-99.02%),并且在8412-8414核苷酸位置缺失三联体密码子GCA,导致甘氨酸氨基酸(Gly2761)缺失。对CP基因序列进行的系统发育分析显示,存在4个分支,分别命名为A、B、C和D,以及最近引入的一个分支B1。所有塞尔维亚小麦WSMV分离株与其他欧洲分离株和一个来自伊朗的分离株一起归为B支。这项研究的结果首次揭示了塞尔维亚WSMV分离株的分子特征,表明它们与其他欧洲分离株关系密切,并且在该国存在单一基因型。系统发育分析也证实了WSMV分离株从单一位点在整个欧洲传播。
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Occurrence and molecular characterization of wheat streak mosaic virus in wheat in Serbia
The wheat streak mosaic virus (WSMV), vectored by the wheat curl mite, is globally distributed and threatens wheat production worldwide. Since its first occurrence in Serbia in the 1960s, WSMV presence has not been monitored. In 2019, a total of 62 samples of fi ve wheat cultivars from eight locations in Serbia were collected and tested for the presence of nine common wheat viruses: WSMV, barley yellow dwarf virus-PAV, -MAV, -SGV, and -RMV, cereal yellow dwarf virus-RPV, wheat spindle streak virus, brome mosaic virus, and soil-borne wheat mosaic virus, using individual or multiplex RT-PCR. WSMV was detected in 58.1% of the tested samples in seven wheat crops at five different locations. Species-specific primers failed to detect the presence of the other eight tested viruses. For further confirmation of WSMV, RT-PCR with the WS8166F/WS8909R primers covering the coat protein (CP) gene was carried out for both amplification and sequencing. The amplified product of the correct predicted size (750 bp) derived from four selected isolates, 98-19, 99-19, 102-19 and 120-19, was sequenced and deposited in GenBank (MT461299, MT461300, MT461301 and MT461302, respectively). Serbian WSMV isolates showed very high nucleotide identity (98.16-99.02%) and shared a deletion of triplet codon GCA at nucleotide position 8412- 8414 resulting in deletion of glycine amino acid (Gly2761). Phylogenetic analysis conducted on CP gene sequences revealed the existence of four clades, named A, B, C and D, and one recently introduced clade B1. All Serbian wheat WSMV isolates grouped into clade B together with other European isolates and one isolate from Iran. The results of this study provide the first insight into molecular characterisation of Serbian WSMV isolates, indicating their close relationship with other European isolates and existence of a single genotype in the country. Phylogenetic analysis also confirms the dispersal of WSMV isolates throughout Europe from a single locus.
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