桑叶丙酮水提物对草甘膦毒性的体内模型肾脏保护作用及其潜在机制

Olfa Rebai, S. Fattouch, M. Amri
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引用次数: 2

摘要

草甘膦是农达中的活性物质,是世界上使用最广泛的农药,可能作为残留物存在于衍生食品和饮用水中。以往的研究证实,桑叶提取物具有多种药理活性。然而,白桦提取物的肾保护作用及其潜在的分子机制尚不清楚。本研究选用Wistar大鼠(180 ~ 200 g), n=5 ~ 6。对照组患者每日1次腹腔注射生理盐水0.2 ml,连用2周。对照动物接受标准饮食。处理组给予多酚提取物(100 mg/kg, i.p.)或草甘膦(100 mg/kg, i.p.),或共给药(提取物?体重1公斤,草甘膦100毫克公斤?每日1 b.w ., i.p .),直至治疗第15天。采用标准显色法测定乳酸脱氢酶LDH、血尿素、肌酐、一氧化氮浓度。桑叶提取物(MLE)显著减轻了肾脏氧化应激,表现为丙二醛(MDA)和蛋白羰基水平升高,超氧化物歧化酶(SOD)活性下降。MLE似乎还能够通过维持游离铁和Ca2 +稳态来调节改变的生化参数,并调节内源性抗氧化酶系统。似乎同时使用富含绿原酸及其异构体的白支原草的丙酮水溶液可以保护肾脏免受草甘膦引起的肾毒性。总的来说,MLE可能对草甘膦诱导的毒性具有保护作用,这可能归因于绿原酸及其异构体,其提取物中最丰富的酚酸。桑叶是酚类化合物的来源,可以是一个很好的开始,以发现一种新的化学化合物,可能导致新的药物。桑树提取物补充剂可以作为开发一种安全的、有前途的肾毒性管理营养保健品的候选物。
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Nephroprotective effect of aqueous acetonic extract of Morus alba and its underlying mechanisms against glyphosate-induced toxicity - in vivo model
Glyphosate, the active substance in RoundupR, is the most widely used pesticide in the world and may be present as a residue in derived foods and drinking water. Previous reports have confirmed that extracts from leaves of Morus alba exert many pharmacological activities. However, renoprotective effects of M. alba extract and its underling molecular mechanism is still unknown. Wistar rats (180-200 g) were used in this study (n=5-6). A control group received 0.2 ml normal saline intraperitoneally (i.p) once daily for two weeks. Control animals received standard diet. Treated groups received either polyphenolic extract (100 mg/kg,i.p) or glyphosate (100 mg/kg, i.p), or co-administration (extract ?g ml?1 kg b.w. and glyphosate 100 mg kg?1 b.w, i.p), daily until the 15thday of treatment. Lactate deshydrogenase LDH, serum concentrations of blood urea, creatinine and nitric oxide were measured using standard coloromertic methods. Renal oxidative stress, evidenced by increased malondialdehyde (MDA) and protein carbonyl levels and decline in superoxide dismutase (SOD) activity, was significantly alleviated by mulberry leaves extract (MLE) administration. MLE also appears to be able to modulate altered biochemical parametres by maintaining free iron and Ca2 + homeostasis, and regulate the endogenous antioxidant enzymes system. It seems that concurrent use of the aqueous acetonic fraction of M. alba, rich in chlorogenic acid and its isomeres, can protect kidneys from glyphosate-induced nephrotoxicity. Overall, MLE may possess protective activity against glyphosate-induced toxicity, which may be attributed to chlorogenic acid and its isomers, the most abundant phenolic acids present in its extracts. Mulberry leaves are a source of phenolic compounds and can be a good start towards discovering a new chemical compound which may lead to a new drug. A mulberry extract supplement could serve as a candidate for developing a safe, and promising nutraceutical product for the management of nephrotoxicity.
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