乳酸菌、光养菌和酵母混合菌不同组合防治番茄和辣椒种子和幼苗病原菌的应用

D. Ristic, I. Vučurović, G. Aleksić, B. Nikolić, Sanja Djurovic, M. Starović
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引用次数: 1

摘要

用3种标记为:EM1、EM5和EM AGRO的甘蔗糖蜜组合(植物乳杆菌、鼠李糖乳杆菌)、光养细菌(红假单胞菌)和酵母(酿酒酵母)对番茄和辣椒的病原菌镰刀菌、alternnaria alternata、Botrytis cinerea、Colletotrichum sp.、Verticilium dahliae和pyium aphanidermatum)进行体外和体内评价。在双琼脂扩散试验中,细菌和酵母的组合EM5对灰绿杆菌的生长抑制率最高(38.4%)。在微稀释试验中,EM1对灰孢杆菌的抑制效果最高(MIC为1 × 10-3 μ l/ml),而EM5对尖孢镰刀菌(F. oxysporum)、alternata和炭黑病菌(Colletotrichum sp. MIC为10 μ l/ml)的抑制效果相似。建议使用EM1(浓度为10和100 μ l/ml)和emagro(浓度为10 μ l/ml)保护番茄幼苗。??1 (100l /ml), ??5和??辣椒幼苗保护推荐使用AGRO (10 ?l/ml)。
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Application of different combinations of lactic acid, phototrophic bacteria and yeast mixtures in control of seed and seedlings pathogens of tomato and pepper
Application of three combinations of lactic acid bacteria (Lactobacillus plantarum, Lactobacillus rhamnosus), phototrophic bacteria (Rhodopseudomonas palustris) and yeast (Saccharomyces cerevisiae) with sugar cane molasses, marked as: EM1, EM5 and EM AGRO, against the phytopathogenic fungi of tomato and pepper: Fusarium oxysporum, Alternaria alternata, Botrytis cinerea, Colletotrichum sp., Verticilium dahliae and Pythium aphanidermatum was evaluated in vitro and in vivo. A combination of bacteria and yeast named EM5 showed the highest mycelium growth inhibition against B. cinerea (38.4%) in a double agar diffusion test. In a microdilution test, the combination EM1 showed the highest inhibitory effect on B. cinerea (MIC 1x10-3 ?l/ml), while EM5 showed a similar inhibitory effect towards F. oxysporum, A. alternata and Colletotrichum sp. (MIC 10 ?l/ml). The use of EM1 (in concentrations 10 and 100 ?l/ml) and EM AGRO (10 ?l/ml) is recommended for tomato seedling protection. ??1 (100 ?l/ml), ??5 and ?? AGRO (10 ?l/ml) are recommended for pepper seedling protection.
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