巨桉早花相关分子标记的鉴定

IF 0.1 0 LITERATURE, ROMANCE Revista do Instituto Florestal Pub Date : 2012-12-12 DOI:10.24278/2178-5031.2012242386
A. Farro, Tânia M. Bortoloto, S. Oda, E. Mello, C. Marino
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引用次数: 1

摘要

开花时间是树木育种的一个重要特征,因为它决定了世代更替的速度,从而决定了遗传增益的速度,这对桉树物种特别感兴趣。利用简单序列重复序列(SSRs)、随机扩增多态性DNA (RAPD)和开花特异性标记对大桉(Eucalyptus grandis)全兄弟姐妹家族的早期开花分离进行了分析,并鉴定了与开花时间控制相关的分子标记。正常开花树(野生型)与早花树杂交得到118个子代,开花时间孟德尔分离比为1:1 (χ2 = 0.5424, P > 0.05),表明一个主基因在大桉(Eucalyptus grandis, PFEg)早花位点上起作用。SSR标记embra02与QTL PFEg相关,确定该区域为性状控制的候选区域。这些图谱可以作为研究的基础,在研究中可以插入新的标记,试图找到更多与桉树早花特征相关的位点。
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IDENTIFICATION OF MOLECULAR MARKERS LINKED TO EARLY FLOWERING IN Eucalyptus grandis
Flowering time is an important trait for tree breeding because it determines the speed of generation turnover and therefore the rapidity of genetic gains, and it is of particular interest in Eucalyptus species. In this work, we used simple sequence repeats (SSRs), random amplified polymorphic DNA (RAPD) analysis and specific markers for flowering to evaluate early flowering segregation in a full-sibling family of Eucalyptus grandis and to identify molecular markers associated with the control of flowering time. A cross between a normal flowering tree (wild-type) and early flowering tree resulted in 118 progeny with a 1:1 Mendelian segregation ratio for flowering time (χ2 = 0.5424, P > 0.05), which suggested the action of one main gene in a locus named Early flowering in Eucalyptus grandis (PFEg). The SSR marker EMBRA 02 was related to the QTL PFEg, and identified this region as a candidate for trait control. These maps may be used as the basis for a study in which can be inserted new markers in an attempt to find more loci related to early flowering characteristic on eucalyptus.
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