大气压等离子体氮处理诱导成骨细胞分化,降低iNOS和COX-2表达

IF 0.3 4区 医学 Q4 ENGINEERING, BIOMEDICAL Journal of Hard Tissue Biology Pub Date : 2021-01-01 DOI:10.2485/JHTB.30.131
Ryoichi Sato, Yasuhiro Namura, N. Tanabe, M. Sakai, A. Utsu, Keiko Tomita, N. Suzuki, M. Motoyoshi
{"title":"大气压等离子体氮处理诱导成骨细胞分化,降低iNOS和COX-2表达","authors":"Ryoichi Sato, Yasuhiro Namura, N. Tanabe, M. Sakai, A. Utsu, Keiko Tomita, N. Suzuki, M. Motoyoshi","doi":"10.2485/JHTB.30.131","DOIUrl":null,"url":null,"abstract":": In recent years, atmospheric pressure plasma jet (APPJ) has been widely developed for various medical applica-tions, such as medical equipment sterilization, gene transfection, cell proliferation, and wound healing. In particular, non-thermal APPJ enables direct treatment of the biological system without any thermal-associated damage. The effect of cells on APPJ depends upon the gas species used in the treatment. However, the mechanisms underlying osteoblast differen tiation mediated by APPJ with nitrogen are yet to be studied. This study investigated the effects of nitrogen-APPJ on osteo blast differentiation by assessing the transcription factors, extracellular matrix proteins (ECMPs), alkaline phosphatase (ALP) activity, and the mRNA and protein expressions of ALP, inducible nitric oxide synthase (iNOS), and cyclooxygen-ase-2 (COX-2), in an osteoblast mouse cell line. We found that nitrogen-APPJ induced osteoblast differentiation-related transcription factors (runt-related transcription factor 2 [Runx2] and osterix), osteocalcin (OCN), and ALP activity, as well as reduced the mRNA and protein expressions of iNOS and COX-2. Thus, we concluded that APPJ affects differentiation of the osteoblast cells.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.3000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Atmospheric Pressure Plasma Treatment with Nitrogen Induces Osteoblast Differentiation and Reduces iNOS and COX-2 Expressions\",\"authors\":\"Ryoichi Sato, Yasuhiro Namura, N. Tanabe, M. Sakai, A. Utsu, Keiko Tomita, N. Suzuki, M. Motoyoshi\",\"doi\":\"10.2485/JHTB.30.131\",\"DOIUrl\":null,\"url\":null,\"abstract\":\": In recent years, atmospheric pressure plasma jet (APPJ) has been widely developed for various medical applica-tions, such as medical equipment sterilization, gene transfection, cell proliferation, and wound healing. In particular, non-thermal APPJ enables direct treatment of the biological system without any thermal-associated damage. The effect of cells on APPJ depends upon the gas species used in the treatment. However, the mechanisms underlying osteoblast differen tiation mediated by APPJ with nitrogen are yet to be studied. This study investigated the effects of nitrogen-APPJ on osteo blast differentiation by assessing the transcription factors, extracellular matrix proteins (ECMPs), alkaline phosphatase (ALP) activity, and the mRNA and protein expressions of ALP, inducible nitric oxide synthase (iNOS), and cyclooxygen-ase-2 (COX-2), in an osteoblast mouse cell line. We found that nitrogen-APPJ induced osteoblast differentiation-related transcription factors (runt-related transcription factor 2 [Runx2] and osterix), osteocalcin (OCN), and ALP activity, as well as reduced the mRNA and protein expressions of iNOS and COX-2. Thus, we concluded that APPJ affects differentiation of the osteoblast cells.\",\"PeriodicalId\":16040,\"journal\":{\"name\":\"Journal of Hard Tissue Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.3000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Hard Tissue Biology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.2485/JHTB.30.131\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hard Tissue Biology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.2485/JHTB.30.131","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 1

摘要

近年来,大气压等离子体射流(APPJ)在医疗器械灭菌、基因转染、细胞增殖、伤口愈合等方面得到了广泛的应用。特别是,非热APPJ可以直接处理生物系统,而不会产生任何与热相关的损伤。细胞对APPJ的影响取决于治疗中使用的气体种类。然而,氮介导的APPJ诱导成骨细胞分化的机制尚不清楚。本研究通过测定成骨小鼠细胞系的转录因子、细胞外基质蛋白(ECMPs)、碱性磷酸酶(ALP)活性以及ALP、诱导型一氧化氮合酶(iNOS)和环氧化酶-2 (COX-2) mRNA和蛋白表达,探讨氮- appj对成骨细胞分化的影响。我们发现氮- appj诱导成骨细胞分化相关转录因子(runt相关转录因子2 [Runx2]和osterix)、骨钙素(OCN)和ALP活性,并降低iNOS和COX-2 mRNA和蛋白表达。因此,我们认为APPJ影响成骨细胞的分化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Atmospheric Pressure Plasma Treatment with Nitrogen Induces Osteoblast Differentiation and Reduces iNOS and COX-2 Expressions
: In recent years, atmospheric pressure plasma jet (APPJ) has been widely developed for various medical applica-tions, such as medical equipment sterilization, gene transfection, cell proliferation, and wound healing. In particular, non-thermal APPJ enables direct treatment of the biological system without any thermal-associated damage. The effect of cells on APPJ depends upon the gas species used in the treatment. However, the mechanisms underlying osteoblast differen tiation mediated by APPJ with nitrogen are yet to be studied. This study investigated the effects of nitrogen-APPJ on osteo blast differentiation by assessing the transcription factors, extracellular matrix proteins (ECMPs), alkaline phosphatase (ALP) activity, and the mRNA and protein expressions of ALP, inducible nitric oxide synthase (iNOS), and cyclooxygen-ase-2 (COX-2), in an osteoblast mouse cell line. We found that nitrogen-APPJ induced osteoblast differentiation-related transcription factors (runt-related transcription factor 2 [Runx2] and osterix), osteocalcin (OCN), and ALP activity, as well as reduced the mRNA and protein expressions of iNOS and COX-2. Thus, we concluded that APPJ affects differentiation of the osteoblast cells.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Hard Tissue Biology
Journal of Hard Tissue Biology ENGINEERING, BIOMEDICAL-
CiteScore
0.90
自引率
0.00%
发文量
28
审稿时长
6-12 weeks
期刊介绍: Information not localized
期刊最新文献
Evaluation of Maxillary First Molar Intrusion Mechanics with Mini-Implant Anchorages Using the Finite Element Method Study on the Effect of Soft-Start Light on Microleakage in Pit and Fissure Closure Upregulation of miR-101-3p Overcomes Ibrutinib Resistance by Targeting ABCC5 in Diffuse Large B-Cell Lymphoma (DLBCL) miR-141 Improve Osteoporosis by Promoting Osteoblast Differentiation through Targeting RICTOR A Study of Submandibular Gland Changes in Mice of a Murine Model of Sjögren’s Syndrome Administered Dental Pulp Stem Cell-Conditioned Medium
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1