大气压等离子体氮处理诱导成骨细胞分化,降低iNOS和COX-2表达

IF 0.3 4区 医学 Q4 ENGINEERING, BIOMEDICAL Journal of Hard Tissue Biology Pub Date : 2021-01-01 DOI:10.2485/JHTB.30.131
Ryoichi Sato, Yasuhiro Namura, N. Tanabe, M. Sakai, A. Utsu, Keiko Tomita, N. Suzuki, M. Motoyoshi
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引用次数: 1

摘要

近年来,大气压等离子体射流(APPJ)在医疗器械灭菌、基因转染、细胞增殖、伤口愈合等方面得到了广泛的应用。特别是,非热APPJ可以直接处理生物系统,而不会产生任何与热相关的损伤。细胞对APPJ的影响取决于治疗中使用的气体种类。然而,氮介导的APPJ诱导成骨细胞分化的机制尚不清楚。本研究通过测定成骨小鼠细胞系的转录因子、细胞外基质蛋白(ECMPs)、碱性磷酸酶(ALP)活性以及ALP、诱导型一氧化氮合酶(iNOS)和环氧化酶-2 (COX-2) mRNA和蛋白表达,探讨氮- appj对成骨细胞分化的影响。我们发现氮- appj诱导成骨细胞分化相关转录因子(runt相关转录因子2 [Runx2]和osterix)、骨钙素(OCN)和ALP活性,并降低iNOS和COX-2 mRNA和蛋白表达。因此,我们认为APPJ影响成骨细胞的分化。
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Atmospheric Pressure Plasma Treatment with Nitrogen Induces Osteoblast Differentiation and Reduces iNOS and COX-2 Expressions
: In recent years, atmospheric pressure plasma jet (APPJ) has been widely developed for various medical applica-tions, such as medical equipment sterilization, gene transfection, cell proliferation, and wound healing. In particular, non-thermal APPJ enables direct treatment of the biological system without any thermal-associated damage. The effect of cells on APPJ depends upon the gas species used in the treatment. However, the mechanisms underlying osteoblast differen tiation mediated by APPJ with nitrogen are yet to be studied. This study investigated the effects of nitrogen-APPJ on osteo blast differentiation by assessing the transcription factors, extracellular matrix proteins (ECMPs), alkaline phosphatase (ALP) activity, and the mRNA and protein expressions of ALP, inducible nitric oxide synthase (iNOS), and cyclooxygen-ase-2 (COX-2), in an osteoblast mouse cell line. We found that nitrogen-APPJ induced osteoblast differentiation-related transcription factors (runt-related transcription factor 2 [Runx2] and osterix), osteocalcin (OCN), and ALP activity, as well as reduced the mRNA and protein expressions of iNOS and COX-2. Thus, we concluded that APPJ affects differentiation of the osteoblast cells.
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来源期刊
Journal of Hard Tissue Biology
Journal of Hard Tissue Biology ENGINEERING, BIOMEDICAL-
CiteScore
0.90
自引率
0.00%
发文量
28
审稿时长
6-12 weeks
期刊介绍: Information not localized
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