三氧化二砷和四氧化二砷减弱3-甲基胆蒽诱导的人角质形成细胞的细胞毒性

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY Indian Journal of Pharmaceutical Sciences Pub Date : 2023-01-01 DOI:10.36468/pharmaceutical-sciences.1147
J. Kim, Ildoo Kim
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引用次数: 0

摘要

砷化合物减弱3-甲基胆蒽诱导的细胞毒性作为致癌类金属的复杂混合物,砷化合物被报道具有抗细胞毒性和抗肿瘤作用。在这项研究中,我们评估了砷化合物四氧化二砷和三氧化二砷对3-甲基胆碱诱导的人角化细胞毒性的体外保护作用。用不同浓度的砷化合物单独或与3-甲基胆蒽联合处理人角质形成细胞。砷化合物处理不显著影响细胞活力,而3-甲基胆蒽显著降低人角质形成细胞的活力。此外,四氧化二砷和三氧化二砷均可降低3-甲基胆蒽处理的人角质形成细胞中信使核糖核酸和蛋白水平的细胞色素P4501A1的表达。此外,这些砷化合物增加了烟酰胺腺嘌呤二核苷酸磷酸醌氧化还原酶1的表达,该酶被3-甲基胆蒽抑制。综上所述,四氧化二砷和四氧化二砷通过降低细胞色素P4501A1的表达和增加烟酰胺腺嘌呤二核苷酸磷酸醌氧化还原酶1的表达,显著抑制3-甲基胆碱诱导的人角化细胞毒性。此外,四氧化二砷被发现比三氧化二砷更有效地对抗3-甲基胆碱诱导的体外细胞毒性。
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Arsenic Compounds Arsenic Trioxide and Tetraarsenic Oxide Attenuate 3-Methylcholanthrene-Induced Cytotoxicity in Human Keratinocytes
Kim et al. : Arsenic Compounds Attenuate 3-Methylcholanthrene-Induced Cytotoxicity As complex mixtures of carcinogenic metalloids, arsenic compounds have been reported to possess anticytotoxic and antitumor effects. In this study, we evaluated the in vitro protective effects of arsenic compounds tetraarsenic oxide and arsenic trioxide against 3-methylcholanthrene-induced toxicity in human keratinocytes. Human keratinocytes were treated with varying concentrations of arsenic compounds alone or in combination with 3-methylcholanthrene. Treatment with arsenic compounds did not significantly affect cell viability, whereas, 3-methylcholanthrene significantly reduced the viability of human keratinocytes. Furthermore, both tetraarsenic oxide and arsenic trioxide decreased the expression of cytochrome P4501A1 at messenger ribonucleic acid and protein levels in human keratinocytes cells treated with 3-methylcholanthrene. In addition, these arsenic compounds increased the expression of nicotinamide adenine dinucleotide phosphate quinone oxidoreductase 1, which was shown to be inhibited by 3-methylcholanthrene treatment. Together, these findings suggest that tetraarsenic oxide and tetraarsenic oxide significantly inhibit 3-methylcholanthrene-induced cytotoxicity in human keratinocytes by decreasing the expression of cytochrome P4501A1 and increasing the expression of nicotinamide adenine dinucleotide phosphate quinone oxidoreductase 1. Additionally, tetraarsenic oxide was found to be more effective than arsenic trioxide against 3-methylcholanthrene-induced cytotoxicity in vitro .
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期刊介绍: The Indian Journal of Pharmaceutical Sciences (IJPS) is a bi-monthly Journal, which publishes original research work that contributes significantly to further the scientific knowledge in Pharmaceutical Sciences (Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Therapeutics, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Pharmacovigilance, Pharmacoepidemiology, Pharmacoeconomics, Drug Information, Patient Counselling, Adverse Drug Reactions Monitoring, Medication Errors, Medication Optimization, Medication Therapy Management, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest). The Journal publishes original research work either as a Full Research Paper or as a Short Communication. Review Articles on current topics in Pharmaceutical Sciences are also considered for publication by the Journal.
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