补充生物素改变拟南芥根系结构和发育

IF 1 Q3 PLANT SCIENCES Plant Root Pub Date : 2019-01-01 DOI:10.3117/plantroot.13.29
L. F. Ruíz-Herrera, María G. Hernández-Equihua, Daniel Boone-Villa, Gema C. Manuel Jacobo, Asdrúbal Aguilera-Méndez
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引用次数: 1

摘要

生物素是维生素b复合物家族的一员,作为羧化酶的辅助因子,在所有生物体的新陈代谢中都是必不可少的。生物素除了具有重要的代谢功能外,还被认为在调节植物和动物的基因表达方面起着关键作用。然而,所有的植物研究都是在生物素缺乏的情况下进行的。因此,我们假设外源供应的生物素也具有与动物相似的非酶学功能,调节拟南芥的根生长和发育。为了验证这一假设,我们评估了生物素供应对幼苗的影响,并分析了根系构型。补充生物素抑制根伸长,促进侧根形成,并呈浓度依赖性。初生根伸长受到抑制与细胞周期基因CycB1;1:uidA和细胞扩增基因pAtExp7::uidA表达降低相关,并与生物素浓度有关。用AtHisH2B:YFP进行活力测试显示分生组织被耗尽。然而,添加生物素对叶绿素浓度没有影响,但对叶片生长有轻微的抑制作用,并增加了初生根顶端活性氧(ROS)的产生。我们的研究首次证明了生物素在植物中除了作为酶辅助因子的催化作用外,还具有补充生物素的功能,这可能会促进我们对生物素生物学功能的理解。
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Biotin supplementation alters root system architecture and development in Arabidopsis thaliana
Biotin is a member of the vitamin Bcomplex family that acts as a cofactor of carboxylases and is essential in the metabolism of all organisms. In addition to its essential metabolic functions, biotin has been suggested to play a critical role in regulating gene expression in plants and animals. However, all studies in plants have been conducted under biotin deficiency. Therefore, we hypothesized that exogenously supplied biotin also exerts non-enzymological functions similar to those reported in animals, regulating root growth and development in Arabidopsis thaliana. To test this hypothesis, we evaluated the effects of the biotin supply on seedlings and analyzed the root architecture. Biotin supplementation inhibited root elongation and promoted lateral root formation in a concentration-dependent manner. Inhibited primary root elongation was correlated with decreased expression of the cell cycle genes CycB1;1:uidA and cell expansion gene pAtExp7::uidA, and depended on the concentration of biotin. Viability tests with AtHisH2B:YFP showed that the meristem was depleted. However, biotin supplementation did not affect the concentration of chlorophyll but had a slight inhibitory effect on foliage growth and increased the production of reactive oxygen species (ROS) at the apex of primary roots. Our study provides the first evidence of functions of biotin supplementation in plants in addition to its catalytic role as an enzyme cofactor, likely advancing our understanding of the biological functions of biotin.
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来源期刊
Plant Root
Plant Root PLANT SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
期刊介绍: Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.
期刊最新文献
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