{"title":"甘薯不定根特异性基因表达启动子的鉴定","authors":"N. Tanabe, Akane Ito, M. Tamoi, S. Shigeoka","doi":"10.3117/PLANTROOT.12.31","DOIUrl":null,"url":null,"abstract":"Since sweet potato (Ipomoea batatas) produces high yield of storage roots (SR), it is an attractive target for improving productivity. However, molecular information about sweet potato is limited. The number of SR per plants determines the yield of sweet potato. SRs develop from adventitious roots (AR). Therefore, the mechanisms responsible for the initiation and development of AR need to be elucidated to increase the productivity of sweet potato. We conducted a transcriptomic analysis between nodes containing AR primordia (ARnode) and stems using next-generation sequencing to identify AR-specific promoters. A total of 6,219 contigs exhibited stronger expression in the ARnodes than in the stems. Among them, we found that the expression of sweet potato Plant AT-rich sequenceand zinc-binding protein (IbPLATZ) transcription factors was AR-specific. We examined the promoter activity of IbPLATZ in the transgenic Arabidopsis plants. β-glucuronidase (GUS) staining showed that the IbPLATZ promoter conferred the expression of the GUS reporter gene in a root tipspecific manner. These results indicate that the IbPLATZ promoter is available for a root tip-specific foreign gene expression system in transgenic plants.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.12.31","citationCount":"1","resultStr":"{\"title\":\"Identification of promoter for adventitious root-specific gene expression from sweet potato\",\"authors\":\"N. Tanabe, Akane Ito, M. Tamoi, S. Shigeoka\",\"doi\":\"10.3117/PLANTROOT.12.31\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Since sweet potato (Ipomoea batatas) produces high yield of storage roots (SR), it is an attractive target for improving productivity. However, molecular information about sweet potato is limited. The number of SR per plants determines the yield of sweet potato. SRs develop from adventitious roots (AR). Therefore, the mechanisms responsible for the initiation and development of AR need to be elucidated to increase the productivity of sweet potato. We conducted a transcriptomic analysis between nodes containing AR primordia (ARnode) and stems using next-generation sequencing to identify AR-specific promoters. A total of 6,219 contigs exhibited stronger expression in the ARnodes than in the stems. Among them, we found that the expression of sweet potato Plant AT-rich sequenceand zinc-binding protein (IbPLATZ) transcription factors was AR-specific. We examined the promoter activity of IbPLATZ in the transgenic Arabidopsis plants. β-glucuronidase (GUS) staining showed that the IbPLATZ promoter conferred the expression of the GUS reporter gene in a root tipspecific manner. These results indicate that the IbPLATZ promoter is available for a root tip-specific foreign gene expression system in transgenic plants.\",\"PeriodicalId\":20205,\"journal\":{\"name\":\"Plant Root\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2018-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3117/PLANTROOT.12.31\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Root\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3117/PLANTROOT.12.31\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Root","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3117/PLANTROOT.12.31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 1
摘要
由于甘薯(Ipomoea batatas)储藏根(SR)产量高,它是一个有吸引力的提高生产力的目标。然而,关于红薯的分子信息有限。单株SR的多少决定了甘薯的产量。SRs由不定根(AR)发展而来。因此,为了提高甘薯的产量,需要阐明AR的产生和发展机制。我们利用下一代测序技术对含有AR原基(ARnode)的节点和茎进行了转录组学分析,以鉴定AR特异性启动子。6219个contigs在ARnodes中的表达强于在茎中的表达。其中,我们发现甘薯植物AT-rich sequenceand zinc-binding protein (IbPLATZ)转录因子的表达具有ar特异性。我们检测了转基因拟南芥植株中IbPLATZ启动子的活性。β-葡萄糖醛酸酶(GUS)染色表明IbPLATZ启动子以根尖特异性的方式赋予GUS报告基因的表达。这些结果表明IbPLATZ启动子可用于转基因植物根尖特异性外源基因的表达系统。
Identification of promoter for adventitious root-specific gene expression from sweet potato
Since sweet potato (Ipomoea batatas) produces high yield of storage roots (SR), it is an attractive target for improving productivity. However, molecular information about sweet potato is limited. The number of SR per plants determines the yield of sweet potato. SRs develop from adventitious roots (AR). Therefore, the mechanisms responsible for the initiation and development of AR need to be elucidated to increase the productivity of sweet potato. We conducted a transcriptomic analysis between nodes containing AR primordia (ARnode) and stems using next-generation sequencing to identify AR-specific promoters. A total of 6,219 contigs exhibited stronger expression in the ARnodes than in the stems. Among them, we found that the expression of sweet potato Plant AT-rich sequenceand zinc-binding protein (IbPLATZ) transcription factors was AR-specific. We examined the promoter activity of IbPLATZ in the transgenic Arabidopsis plants. β-glucuronidase (GUS) staining showed that the IbPLATZ promoter conferred the expression of the GUS reporter gene in a root tipspecific manner. These results indicate that the IbPLATZ promoter is available for a root tip-specific foreign gene expression system in transgenic plants.
期刊介绍:
Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.