大白菜与紫甘蓝种间杂交种的生产与鉴定

H. Qiao, Fei Li, Shujiang Zhang, Shifan Zhang, Hui Zhang, R. Sun
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引用次数: 2

摘要

北京芸苔不同品种种间杂交研究奥尔森(Olsson)和变种甘蓝(芸苔)进行了试验。杂种是通过连续的芽授粉和胚珠培养获得的,并通过细胞学鉴定进行了检验。结果表明,北京刺花甘蓝与花椒甘蓝种间杂交得到57株,细胞学鉴定和花粉性状调查表明,47株杂种具有预期的染色体(2n=19),花粉不育;6株杂种具有38条染色体,可育。利用可育杂种F1与北京拉帕回交获得BC1植株,田间调查结果表明,所有F1杂种均具有亲本的许多性状,BC1植株抽头明显,与北京拉帕相似。
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Production and Identification of Interspecific Hybrids between Chinese Cabbage and Purple Cabbage
Interspecific hybridizations between different varieties of Brassica rapa L.ssp.peki-nensis(Lour.) Olsson and Brassica oleracea var.capitata L.were carried out.The hybrids were obtained by successive bud pollination and ovule culture and tested by means of identification by cytology.Results showed that 57 plants were obtained by interspecific hybridization between B.rapa L.ssp.pekinensis and B.oleracea var.capitata L.Cytological identification and pollen characteristics survey revealed that 47 of the hybrids had the anticipated chromosomes(2n=19) and pollen fertility was aborted;while 6 hybrids had 38chromosomes and were fertile.The BC1 plants were obtained through back cross between fertile hybrids F1 and B.rapa L.ssp.pekinensis.Field investigation showed that all F1 hybrids had many characteristics from both parents,the BC1 plants had obvious heading and were similar to B.rapa L.ssp.pekinensis.
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