Influence of humic substances on physiological osteogenesis and the blood system of rabbits while using PLA implants

PLA-based implants are characterized by high biocompatibility with body tissues, and are also distinguished by their biodegradable and osseointegrative properties. The regulation of bone tissue growth and development is inextricably linked with the blood system, which is a source of plastic substances and hormones of calcium homeostasis. When PLA implants were inserted into the parietal bone of rabbits, changes occurred in their bodies that were typical for the period after orthopedic and traumatological operations. Metabolism dysregulation of macro- and microelements and disturbances of erythropoiesis, which can occur in the postoperative period, have a negative effect on the bone tissue growth in the young rabbits’ body. It has been proven that humic substances were able to influence the activity of osteoblasts and regulate mineral metabolism. For the experiment, 32 rabbits were divided into four groups, 8 rabbits in each group; sixteen rabbits underwent surgery using PLA implants, which were placed bilaterally in the parietal bone. Eight of the 16 operated rabbits were given Humilid together with water, the rest of them received pure water. Eight of 16 rabbits, intact before surgery, received Humilid together with water, the non-surgical control group did not receive Humilid. The influence of humic substances on physiological osteogenesis and hematological parameters during the use of PLA implants was determined. On the 14th day of the experiment, the rabbits of the surgical group receiving Humilid did not show a decrease in erythrocytes, hemoglobin, and an increase in leukocytes, in contrast to the rabbits of the surgical control group. An increase in the number of osteons and osteoblasts layers was recorded in the femur of the young rabbits that were receiving Humilid, and in the sternum – the number of osteons and layers of osteoblasts. There was also an increase in the number of erythrocytes and hemoglobin in the non-surgical group rabbits that were receiving Humilid to compare the rabbits of the non-surgical control group. ln rabbits that received Humilid, there was an intensification of bone tissue growth against the background of physiological and reparative osteogenesis.