The Increase of Madura Cows Reproduction Performance with Double-Dose Method of Artificial Insemination

: One of the potencies of Madura cow genetics is its adaptation ability to the tropical climate. Yet in most cases, the reproduction efficiency of the Madura cows has not reached the optimum point. This research examines the use of double-dose artificial insemination compared to a single-dose on Madura cows. The study was conducted in Daramista, Lenteng District, Sumenep Regency, Madura, East Java. This research utilized an experimental field method with purposive sampling on 100 Madura cows, with a body condition score of 3-4, aged about 2-6 years old. The treatments were divided into two groups, T0 and T1. T0 cows received single-dose artificial insemination treatment on the 8 th h after estrus and T1 cows received double-dose artificial insemination treatment on the 2 nd and 8 th h after estrus. The Singosari National Artificial Insemination Centre produced Madura bull frozen semen straws used in this research. Before inseminating, frozen semen straws were thawed at 28°C for 30 sec. This research observed some variables, including; Non-Return Rate (NRR1, NRR2, NRR3), Service per Conception (S/C), Conception Rate (CR), and estrus quality condition as supporting data. The researchers analyzed the data descriptively and through chi-square analysis. The result of the study showed that NRR1, NRR2, and NRR3 of T0 were 94, 84, and 70%, while NRR1, NRR2, and NRR3 T1 were 92, 86, and 82%. The CR of T0 was 64% compared to the CR of T1 at 76%. The double dose insemination method at different times on the 2 nd and 8 th h after estrus can increase the reproduction performance in Madura cows.