将风味包封在脂性耶氏菌活性酵母细胞中。休克过程中脂滴形态和甾醇/甾醇平衡的荧光研究

IF 1.1 Q4 BIOPHYSICS AIMS Biophysics Pub Date : 2022-01-01 DOI:10.3934/biophy.2022022
T. Ta, Cynthia Romero-Guido, Thi Hanh Phan, H. D. Tran, H. T. Dinh, Y. Waché
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引用次数: 1

摘要

酵母是包封化合物的强大材料。通常,用作胶囊的酵母被包封处理灭活,这对细胞是有压力的。然而,如果保持活性,细胞除了被包裹的化合物外,还能带来自己的活性。我们以前观察到,脂质培养的多脂耶氏菌更耐包封。本研究的目的是确定与这种抗性有关的生理标记。细胞在葡萄糖或油酸甲酯作为唯一碳源的条件下培养,并接受γ-十二内酯应激。本文主要研究了细胞内脂滴(LDs)和麦角甾醇含量在内酯处理过程中对细胞的保护作用。脂质培养的细胞对内酯的抵抗力更强,而在休克前存在ld显著增加了这种抵抗力。从LD池麦角甾醇酯水解释放麦角甾醇能够加强质膜在休克。对于缺乏lld的细胞,膜麦角甾醇随着lld的生长而酯化,导致膜弱化。通过使用具有大量小尺寸lld的pox3突变株,我们观察到这些突变株的原始行为:这些突变株的抗性没有增加,它们的lld在细胞质中受到冲击时爆炸。这些结果指出了lld在细胞抵抗两亲性应激中的作用,作为一种储存室,以及麦角甾醇的稳态。
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Encapsulation of flavours into Yarrowia lipolytica active yeast cells. Fluorescence study of the lipid droplets morphology and steryl/sterol balance during the shock
Yeast are a powerful material for the encapsulation of compounds. Usually, yeast used as capsules are inactivated by the encapsulation treatment, which is stressful to cells. However, if kept active, cells can bring their own activity in addition to the encapsulated compound. We have observed previously that lipid-grown Yarrowia lipolytica were more resistant to encapsulation. The objective of the present study was to identify physiological markers involved in this resistance. Cells were cultured in the presence of glucose or methyl-oleate as the sole carbon source and submitted to a γ-dodecalactone stress. This paper focuses on the role of intracellular lipid droplets (LDs) and of the ergosteryl content to protect cells during the lactone treatment. Lipid-grown cells were more resistant to lactone and the presence of LDs before the shock increased significantly the resistance. The ergosteryl esters from the LD pool were hydrolysed to release ergosterol able to strenghten the plasma membrane during the shock. For cells devoid of LDs, membrane ergosterols were esterified concomitantly with LDs growth, resulting in a membrane weakening. By using pox3-mutant strains, which possesse numerous and small-sized LDs, we observed the original behaviour: these mutants showed no increased resistance and their LDs exploded in the cytoplasma during the shock. These results point out the role of LDs in cell resistance to amphiphilic stresses as a storage compartment as well as in ergosterol homeostasis.
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来源期刊
AIMS Biophysics
AIMS Biophysics BIOPHYSICS-
CiteScore
2.40
自引率
20.00%
发文量
16
审稿时长
8 weeks
期刊介绍: AIMS Biophysics is an international Open Access journal devoted to publishing peer-reviewed, high quality, original papers in the field of biophysics. We publish the following article types: original research articles, reviews, editorials, letters, and conference reports. AIMS Biophysics welcomes, but not limited to, the papers from the following topics: · Structural biology · Biophysical technology · Bioenergetics · Membrane biophysics · Cellular Biophysics · Electrophysiology · Neuro-Biophysics · Biomechanics · Systems biology
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