M. V. Fipke, A. Balbinot, V. E. Viana, V. R. Gehrke, M. Kemmerich, F. Dayan, G. M. Souza, E. R. Camargo, Luis A. Avila
{"title":"干旱胁迫的跨代记忆和草甘膦的低率降低了稻草对除草剂的敏感性","authors":"M. V. Fipke, A. Balbinot, V. E. Viana, V. R. Gehrke, M. Kemmerich, F. Dayan, G. M. Souza, E. R. Camargo, Luis A. Avila","doi":"10.51694/advweedsci/2022;40:00016","DOIUrl":null,"url":null,"abstract":": Background: Eragrostis plana is the main invasive plant in the Pampa Biome of southern Brazil. This plant is highly competitive, tolerant to abiotic stresses, and very difficult to manage. Plants exposed to abiotic stresses and herbicides develop mechanisms that help alleviate or reduce damage caused by stressors and transmit this ability to the progenies. Objective : The study’s objectives were to ascertain whether acclimatization to stress due to drought and sub-lethal doses of glyphosate may decrease plant sensitivity to glyphosate and investigate the possible memory mechanisms involved in this process. Methods : A population of E. plana was submitted to drought, glyphosate, or a combination of drought plus glyphosate for two generations. The progenies were analyzed for sensitivity to the herbicide glyphosate and for biochemical, metabolites, and molecular responses. Results : When testing sensitivity to glyphosate in the G 2 generations, the DRYxGLY (plants stressed with drought plus glyphosate) was the least sensitive (ED 50 443.0 g a.e. ha -1 ), while CHK (without stresses) was the most sensitive (ED 50 278.0 g a.e. ha -1 ). Evaluating the effects of glyphosate, the CHK population showed a greater accumulation of oxidative damage and shikimic acid. While DRYxGLY had greater antioxidant activity and higher expressions of the EPSPS and ABC-carrier MRP10 genes. Conclusions : Recurrent selection with drought stress and sub-lethal rate of glyphosate (DRYxGLY) showed reduced sensitivity to glyphosate in the second generation ( G 2 ). The conjunction of factors, including the upregulation of EPSPS and the ABC MRP10 transporter, antioxidant enzymes seem to decrease the sensitivity of the DRYxGLY population to glyphosate.","PeriodicalId":29845,"journal":{"name":"Advances in Weed Science","volume":"30 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transgenerational memory of drought stress and low rates of glyphosate reduce the sensitivity of Eragrostis plana to the herbicide\",\"authors\":\"M. V. Fipke, A. Balbinot, V. E. Viana, V. R. Gehrke, M. Kemmerich, F. Dayan, G. M. Souza, E. R. Camargo, Luis A. Avila\",\"doi\":\"10.51694/advweedsci/2022;40:00016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\": Background: Eragrostis plana is the main invasive plant in the Pampa Biome of southern Brazil. This plant is highly competitive, tolerant to abiotic stresses, and very difficult to manage. Plants exposed to abiotic stresses and herbicides develop mechanisms that help alleviate or reduce damage caused by stressors and transmit this ability to the progenies. Objective : The study’s objectives were to ascertain whether acclimatization to stress due to drought and sub-lethal doses of glyphosate may decrease plant sensitivity to glyphosate and investigate the possible memory mechanisms involved in this process. Methods : A population of E. plana was submitted to drought, glyphosate, or a combination of drought plus glyphosate for two generations. The progenies were analyzed for sensitivity to the herbicide glyphosate and for biochemical, metabolites, and molecular responses. Results : When testing sensitivity to glyphosate in the G 2 generations, the DRYxGLY (plants stressed with drought plus glyphosate) was the least sensitive (ED 50 443.0 g a.e. ha -1 ), while CHK (without stresses) was the most sensitive (ED 50 278.0 g a.e. ha -1 ). Evaluating the effects of glyphosate, the CHK population showed a greater accumulation of oxidative damage and shikimic acid. While DRYxGLY had greater antioxidant activity and higher expressions of the EPSPS and ABC-carrier MRP10 genes. Conclusions : Recurrent selection with drought stress and sub-lethal rate of glyphosate (DRYxGLY) showed reduced sensitivity to glyphosate in the second generation ( G 2 ). 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引用次数: 0
摘要
背景:扁Eragrostis plana是巴西南部潘帕草原生物群系的主要入侵植物。这种植物具有很强的竞争力,对非生物胁迫具有耐受性,而且很难管理。暴露于非生物胁迫和除草剂的植物会发展出有助于减轻或减少应激源造成的损害的机制,并将这种能力传递给后代。目的:本研究的目的是确定干旱和亚致死剂量草甘膦胁迫的适应是否会降低植物对草甘膦的敏感性,并探讨这一过程可能涉及的记忆机制。方法:将plana种群置于干旱、草甘膦或干旱加草甘膦的组合下两代。分析了后代对除草剂草甘膦的敏感性以及生化、代谢物和分子反应。结果:在g2代草甘膦敏感性测试中,干旱加草甘膦胁迫植株DRYxGLY对草甘膦敏感性最低(ED 50 443.0 G a.e. ha -1),未胁迫植株CHK对草甘膦敏感性最高(ED 50 278.0 G a.e. ha -1)。评估草甘膦的影响,CHK人群显示出更大的氧化损伤和莽草酸积累。而DRYxGLY具有更强的抗氧化活性,且EPSPS和abc载体MRP10基因的表达量更高。结论:干旱胁迫下的反复选择和草甘膦亚致死率(DRYxGLY)显示第二代对草甘膦的敏感性降低(g2)。包括EPSPS和ABC MRP10转运蛋白上调、抗氧化酶等因素的共同作用似乎降低了DRYxGLY群体对草甘膦的敏感性。
Transgenerational memory of drought stress and low rates of glyphosate reduce the sensitivity of Eragrostis plana to the herbicide
: Background: Eragrostis plana is the main invasive plant in the Pampa Biome of southern Brazil. This plant is highly competitive, tolerant to abiotic stresses, and very difficult to manage. Plants exposed to abiotic stresses and herbicides develop mechanisms that help alleviate or reduce damage caused by stressors and transmit this ability to the progenies. Objective : The study’s objectives were to ascertain whether acclimatization to stress due to drought and sub-lethal doses of glyphosate may decrease plant sensitivity to glyphosate and investigate the possible memory mechanisms involved in this process. Methods : A population of E. plana was submitted to drought, glyphosate, or a combination of drought plus glyphosate for two generations. The progenies were analyzed for sensitivity to the herbicide glyphosate and for biochemical, metabolites, and molecular responses. Results : When testing sensitivity to glyphosate in the G 2 generations, the DRYxGLY (plants stressed with drought plus glyphosate) was the least sensitive (ED 50 443.0 g a.e. ha -1 ), while CHK (without stresses) was the most sensitive (ED 50 278.0 g a.e. ha -1 ). Evaluating the effects of glyphosate, the CHK population showed a greater accumulation of oxidative damage and shikimic acid. While DRYxGLY had greater antioxidant activity and higher expressions of the EPSPS and ABC-carrier MRP10 genes. Conclusions : Recurrent selection with drought stress and sub-lethal rate of glyphosate (DRYxGLY) showed reduced sensitivity to glyphosate in the second generation ( G 2 ). The conjunction of factors, including the upregulation of EPSPS and the ABC MRP10 transporter, antioxidant enzymes seem to decrease the sensitivity of the DRYxGLY population to glyphosate.