三种松子科植物化学筛选及高效薄层色谱指纹图谱

K. Geethika, P. Sunojkumar
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引用次数: 5

摘要

背景:芦荚属植物具有很高的经济潜力。作为草药,这些在阿育吠陀文献中被称为“Droṇapuhṣpī”。本研究旨在对三种Leucas植物进行植物化学筛选和HPTLC指纹图谱分析。材料和方法:对每种植物的水、甲醇、乙醇和氯仿提取液进行定性植物化学筛选。用标准分光光度法测定甲醇提取物中总酚、总黄酮和单宁的含量。建立了高效液相色谱(HPTLC)分离提取液中有效成分的方法,并在默克公司硅胶预涂铝板上采用全自动薄层色谱涂布器,溶剂体系为甲苯:乙酸乙酯:7:3,对甲醇提取液进行了薄层色谱分析。结果:不同提取物的初步植物化学筛选表明,黄酮类、萜烯类、单宁类、碳水化合物、苷类、酚类化合物、蛋白质和氨基酸等植物成分不同。三种提取物中,毛缕草的酚类物质(34.5±0.35)、黄酮(48.25±1.06)和单宁(6.45±0.5)含量最高。甲醇叶提取物的hplc指纹图谱在550 nm处扫描得到12个峰,15个峰,13个峰,Rf值在0.06 ~ 0.98之间。
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Phytochemical screening and high-performance thin-layer chromatography fingerprint profile of three species of leucas (Lamiaceae)
Background: The members of genus Leucas possess high economic potential. As medicinal herbs these were well known as 'Droṇapuhṣpī' in Ayurveda literature. The present study aims to carry out the phytochemical screening as well as the HPTLC fingerprint profiling of three species of Leucas. Materials and Methods: Aqueous, methanol, ethanol and chloroform extracts of each plant were subjected to qualitative phytochemical screening. The total phenols, flavonoids and tannins were quantified in the methanolic extract by standard spectrophotometric methods. HPTLC method for the separation of the active constituents in extracts has been developed and TLC of the methanolic extracts on silica gel pre-coated aluminum plates of Merck by automatic TLC applicator and using solvent system Toluene: ethyl acetate:7:3 was performed. Results: Preliminary phytochemical screening of different extracts showed the presence of different phytoconstituents such as flavonoids, terpenes, tannins, carbohydrates, glycosides, phenolic compounds, proteins and amino acids. Among all the three extracts studied, L. ciliata had the highest concentration of phenolics (34.5 ± 0.35), flavonoids (48.25 ± 1.06) and tannins (6.45 ± 0.5). A HPTLC fingerprint scanned at 550 nm for methanol leaf extracts revealed 12 peaks for L. stelligera, 15 peaks for L. eriostoma and 13 peaks for L. ciliata with Rf values in the range of 0.06 to 0.98.
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