{"title":"姜黄素在衰老过程中改善红细胞的氧化应激","authors":"Akshay Kumar, P. Maurya","doi":"10.56042/ijnpr.v14i1.1127","DOIUrl":null,"url":null,"abstract":"An essential dietary flavonoid known as curcumin has positive health effects and inhibits the synthesis of reactive oxygen species (ROS). The aim of this study is to look at signs of oxidative stress in red blood cells (RBCs) treated with curcumin as a function of age. A total of 116 healthy volunteers ranging in age from 20 to 81 years provided clinically pertinent blood samples for the investigation. Three groups of subjects were created: young (20 to 35 years), middle (36 to 60 years), and old (>60 years). Oxidative stress was induced in vitro by incubating RBCs with 10 -5 M tert -butyl hydroperoxide ( t -BHP). Malondialdehyde and reduced glutathione were detected by co-incubating RBCs with curcumin (10 -8 M to 10 -5 M final concentration) and t -BHP to assess the effect of curcumin. After being incubated with t -BHP, the results revealed higher MDA levels ( P <0.001) in comparison to their respective controls and the GSH level significantly ( P <0.001) decreased during ageing. By raising GSH and lowering MDA levels, curcumin treatment in vitro considerably ( P <0.01) mitigated the harmful effect of oxidative stress in RBCs from all age groups. The results of this study showed the potential role of curcumin in the ageing process and it will facilitate the quick screening of novel chemical compounds that may protect RBCs from oxidative stress.","PeriodicalId":13297,"journal":{"name":"Indian Journal of Natural Products and Resources","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Curcumin ameliorates oxidative stress in red blood cells during ageing\",\"authors\":\"Akshay Kumar, P. Maurya\",\"doi\":\"10.56042/ijnpr.v14i1.1127\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An essential dietary flavonoid known as curcumin has positive health effects and inhibits the synthesis of reactive oxygen species (ROS). The aim of this study is to look at signs of oxidative stress in red blood cells (RBCs) treated with curcumin as a function of age. A total of 116 healthy volunteers ranging in age from 20 to 81 years provided clinically pertinent blood samples for the investigation. Three groups of subjects were created: young (20 to 35 years), middle (36 to 60 years), and old (>60 years). Oxidative stress was induced in vitro by incubating RBCs with 10 -5 M tert -butyl hydroperoxide ( t -BHP). Malondialdehyde and reduced glutathione were detected by co-incubating RBCs with curcumin (10 -8 M to 10 -5 M final concentration) and t -BHP to assess the effect of curcumin. After being incubated with t -BHP, the results revealed higher MDA levels ( P <0.001) in comparison to their respective controls and the GSH level significantly ( P <0.001) decreased during ageing. By raising GSH and lowering MDA levels, curcumin treatment in vitro considerably ( P <0.01) mitigated the harmful effect of oxidative stress in RBCs from all age groups. The results of this study showed the potential role of curcumin in the ageing process and it will facilitate the quick screening of novel chemical compounds that may protect RBCs from oxidative stress.\",\"PeriodicalId\":13297,\"journal\":{\"name\":\"Indian Journal of Natural Products and Resources\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Natural Products and Resources\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.56042/ijnpr.v14i1.1127\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Natural Products and Resources","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.56042/ijnpr.v14i1.1127","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Curcumin ameliorates oxidative stress in red blood cells during ageing
An essential dietary flavonoid known as curcumin has positive health effects and inhibits the synthesis of reactive oxygen species (ROS). The aim of this study is to look at signs of oxidative stress in red blood cells (RBCs) treated with curcumin as a function of age. A total of 116 healthy volunteers ranging in age from 20 to 81 years provided clinically pertinent blood samples for the investigation. Three groups of subjects were created: young (20 to 35 years), middle (36 to 60 years), and old (>60 years). Oxidative stress was induced in vitro by incubating RBCs with 10 -5 M tert -butyl hydroperoxide ( t -BHP). Malondialdehyde and reduced glutathione were detected by co-incubating RBCs with curcumin (10 -8 M to 10 -5 M final concentration) and t -BHP to assess the effect of curcumin. After being incubated with t -BHP, the results revealed higher MDA levels ( P <0.001) in comparison to their respective controls and the GSH level significantly ( P <0.001) decreased during ageing. By raising GSH and lowering MDA levels, curcumin treatment in vitro considerably ( P <0.01) mitigated the harmful effect of oxidative stress in RBCs from all age groups. The results of this study showed the potential role of curcumin in the ageing process and it will facilitate the quick screening of novel chemical compounds that may protect RBCs from oxidative stress.
期刊介绍:
Indian Journal of Natural Products and Resources (IJNPR), a quarterly journal, is dedicated to the exploration of topics that confront both educators, frontline workers in the fields, entrepreneurs and ultimate users of the value added products and their resources pertaining to both plants and animals. It publishes original research papers, reviews and short communications. The scope of the journal is multidisciplinary especially of applied nature. Papers on plant cultivation, fishery, apiary, poultry, dairy, disease and pest control measures, postharvest technology/practices, value added products and utilization of plants and animals in broader sense (including ethnobotany and pharmacology) are considered for publication.