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引用次数: 0
摘要
目的：中枢神经系统的恶性肿瘤，即神经胶质瘤，因其进展迅速而广为人知。与所有恶性肿瘤一样，胶质瘤也是由癌基因刺激和肿瘤抑制因子抑制引起的。最近的研究表明，人类巨细胞病毒（HCMV）参与了胶质瘤的发病机制；因此，我们旨在证明 HCMV 基因组是否是胶质瘤细胞基因组 DNA 的一部分：研究对象包括 U87MG 胶质母细胞瘤细胞培养物和胶质瘤患者的肿瘤样本。提取肿瘤样本和 U87MG 细胞的基因组 DNA，采用实时定量 PCR 方法评估人类巨细胞病毒基因组 DNA 的存在结果：结果：在上述实验条件下，肿瘤和细胞系基因组 DNA 中未检测到 HCMV 阳性：结论：我们发现所有样本的基因组 DNA 均呈阴性。结论：我们发现，所有样本的基因组 DNA 中 HCMV 基因组 DNA 均为阴性，因此在人类胶质瘤肿瘤中检测不到 HCMV。因此，总的病毒 DNA 与胶质瘤的发病机制无关，但小的病毒颗粒或特定基因可能被整合到胶质瘤细胞的基因组 DNA 中，从而导致癌症的发生。这有待进一步研究验证。
Non-Detection of HCMV Total Genomic DNA in Human Glioma Cells Genome.
Aim: To demonstrate if the human cytomegalovirus (HCMV) genome, that is involved in the pathogenesis of gliomas, is part of the genomic DNA of glioma cells or not.
Material and methods: The study included U87MG glioblastoma cell culture and tumor samples from glioma patients. The genomic DNA of tumor samples and U87MG cells were extracted and real-time quantitative PCR was used to assess the presence of the human cytomegalovirus genomic DNA.
Results: Consequently, HCMV positivity was not detected in the tumor and cell line genomic DNA under the aforementioned experimental conditions.
Conclusion: We found that the genomic DNA of all the samples was negative for HCMV genomic DNA. Thus, HCMV could not be detected in human glioma tumors and we put forward that HCMV genomic DNA was not incorporated into the genomic DNA of glioma cells. Thus, total viral DNA is not involved in the pathogenesis of glioma; however, small viral particles or specific genes might be incorporated into the genomic DNA of glioma cells, leading to cancer development. This prompts further studies for verification.
期刊介绍：
Turkish Neurosurgery is a peer-reviewed, multidisciplinary, open access and totally free journal directed at an audience of neurosurgery physicians and scientists. The official language of the journal is English. The journal publishes original articles in the form of clinical and basic research. Turkish Neurosurgery will only publish studies that have institutional review board (IRB) approval and have strictly observed an acceptable follow-up period. With the exception of reference presentation, Turkish Neurosurgery requires that all manuscripts be prepared in accordance with the Uniform Requirements for Manuscripts Submitted to Biomedical Journals.
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