伊拉克多囊卵巢综合征患者KISS1基因变异rs372790354 G b> A和rs4889 G>A对kisspeptin的影响

Q3 Biochemistry, Genetics and Molecular Biology Journal of Advanced Biotechnology and Experimental Therapeutics Pub Date : 2022-01-01 DOI:10.5455/jabet.2022.d136
Nora Musawi, S. Qaysi, S. Witwit
{"title":"伊拉克多囊卵巢综合征患者KISS1基因变异rs372790354 G b> A和rs4889 G>A对kisspeptin的影响","authors":"Nora Musawi, S. Qaysi, S. Witwit","doi":"10.5455/jabet.2022.d136","DOIUrl":null,"url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a heterogeneous genetic disorder categorized by hyperandrogenism that affects early reproductive age in females, KISS1 has play role in regulating the hypothalamic-pituitary-gonad axis, it plays a key role in human reproductive function. In patients with polycystic ovarian syndrome, imbalance-of-function mutations in this gene have been found often. blood samples were collected from 120 patients (60 control are divided into 30 normal weight and 30 obese) and (60 PCOS) females are divided into 30 normal weight and 30 obese). DNA was extracted and genotyped for KISS1 variants by HRM-PCR and measured level of kisspeptin by ELIAS, while LH, FSH, DHEA and free testosterone by CLIA. The value of LH, Testosterone, DHEA-S and kisspeptin is elevated in the patient group, while the decline of FSH in serum level patients value, rs372790354 G > A and rs4889 G>A was associated with PCOS in dominant, recessive, co-dominant (P-value< 0.05), rs37279054 AA was not found the effect of obese group and linked with normal weight PCOS put present study no effect on the parameters, rs4889 GG/GA was the effect on all subgroups except the genotype GA not effected on obese female, the highly significant ( P-value<0.05) of rs4889 GA influenced on measured of WHR, LH/FSH ratio and DHEA-S in the patient compared to control, rs4889 GG/AA was influenced on normal-weight patient compare to an obese patient, the WHR was higher in an obese patient in both genotype. While the level of kisspeptin in normal weight with genotype AA was higher level compared to obese and (P-value<0.05). We concluded that the KISS1 levels were higher in PCOS females compared to controls and decreased with increasing BMI, Kiss1 polymorphism rs372790354 G>A and rs4889 G>A may be associated with the pathophysiology of PCOS and lead to increase serum level of LH that due to hyperandrogenism.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of KISS1 gene variants (rs372790354 G>A and rs4889 G>A) on kisspeptin in patients with polycystic ovary syndrome in Iraq.\",\"authors\":\"Nora Musawi, S. Qaysi, S. Witwit\",\"doi\":\"10.5455/jabet.2022.d136\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Polycystic ovary syndrome (PCOS) is a heterogeneous genetic disorder categorized by hyperandrogenism that affects early reproductive age in females, KISS1 has play role in regulating the hypothalamic-pituitary-gonad axis, it plays a key role in human reproductive function. In patients with polycystic ovarian syndrome, imbalance-of-function mutations in this gene have been found often. blood samples were collected from 120 patients (60 control are divided into 30 normal weight and 30 obese) and (60 PCOS) females are divided into 30 normal weight and 30 obese). DNA was extracted and genotyped for KISS1 variants by HRM-PCR and measured level of kisspeptin by ELIAS, while LH, FSH, DHEA and free testosterone by CLIA. The value of LH, Testosterone, DHEA-S and kisspeptin is elevated in the patient group, while the decline of FSH in serum level patients value, rs372790354 G > A and rs4889 G>A was associated with PCOS in dominant, recessive, co-dominant (P-value< 0.05), rs37279054 AA was not found the effect of obese group and linked with normal weight PCOS put present study no effect on the parameters, rs4889 GG/GA was the effect on all subgroups except the genotype GA not effected on obese female, the highly significant ( P-value<0.05) of rs4889 GA influenced on measured of WHR, LH/FSH ratio and DHEA-S in the patient compared to control, rs4889 GG/AA was influenced on normal-weight patient compare to an obese patient, the WHR was higher in an obese patient in both genotype. While the level of kisspeptin in normal weight with genotype AA was higher level compared to obese and (P-value<0.05). We concluded that the KISS1 levels were higher in PCOS females compared to controls and decreased with increasing BMI, Kiss1 polymorphism rs372790354 G>A and rs4889 G>A may be associated with the pathophysiology of PCOS and lead to increase serum level of LH that due to hyperandrogenism.\",\"PeriodicalId\":36275,\"journal\":{\"name\":\"Journal of Advanced Biotechnology and Experimental Therapeutics\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Advanced Biotechnology and Experimental Therapeutics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/jabet.2022.d136\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Biotechnology and Experimental Therapeutics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/jabet.2022.d136","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0

摘要

多囊卵巢综合征(Polycystic ovarian syndrome, PCOS)是一种影响女性早育年龄的异源性遗传疾病,KISS1在调节下丘脑-垂体-性腺轴中发挥重要作用,在人类生殖功能中起关键作用。在多囊卵巢综合征患者中,经常发现该基因的功能不平衡突变。采集120例患者(对照组60例,体重正常30例,肥胖30例)和PCOS女性60例,体重正常30例,肥胖30例)的血液样本。提取DNA,采用HRM-PCR分型,elisa检测kisspeptin水平,CLIA检测LH、FSH、DHEA和游离睾酮水平。LH的价值,睾酮、硫酸脱氢表雄酮(dhea - s和kisspeptin升高的患者群体,而病人血清中FSH水平的下降值,rs372790354 G >和rs4889 G >主导与PCOS有关,隐性,共显性(p < 0.05),未找到rs37279054 AA的影响肥胖组和正常体重与PCOS把本研究没有对参数的影响,rs4889 GG / GA对所有子组的影响除了基因型遗传算法不影响肥胖的女性,高p值ea和rs4889 G>A可能与PCOS的病理生理有关,并导致高雄激素血症引起的血清LH水平升高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Effect of KISS1 gene variants (rs372790354 G>A and rs4889 G>A) on kisspeptin in patients with polycystic ovary syndrome in Iraq.
Polycystic ovary syndrome (PCOS) is a heterogeneous genetic disorder categorized by hyperandrogenism that affects early reproductive age in females, KISS1 has play role in regulating the hypothalamic-pituitary-gonad axis, it plays a key role in human reproductive function. In patients with polycystic ovarian syndrome, imbalance-of-function mutations in this gene have been found often. blood samples were collected from 120 patients (60 control are divided into 30 normal weight and 30 obese) and (60 PCOS) females are divided into 30 normal weight and 30 obese). DNA was extracted and genotyped for KISS1 variants by HRM-PCR and measured level of kisspeptin by ELIAS, while LH, FSH, DHEA and free testosterone by CLIA. The value of LH, Testosterone, DHEA-S and kisspeptin is elevated in the patient group, while the decline of FSH in serum level patients value, rs372790354 G > A and rs4889 G>A was associated with PCOS in dominant, recessive, co-dominant (P-value< 0.05), rs37279054 AA was not found the effect of obese group and linked with normal weight PCOS put present study no effect on the parameters, rs4889 GG/GA was the effect on all subgroups except the genotype GA not effected on obese female, the highly significant ( P-value<0.05) of rs4889 GA influenced on measured of WHR, LH/FSH ratio and DHEA-S in the patient compared to control, rs4889 GG/AA was influenced on normal-weight patient compare to an obese patient, the WHR was higher in an obese patient in both genotype. While the level of kisspeptin in normal weight with genotype AA was higher level compared to obese and (P-value<0.05). We concluded that the KISS1 levels were higher in PCOS females compared to controls and decreased with increasing BMI, Kiss1 polymorphism rs372790354 G>A and rs4889 G>A may be associated with the pathophysiology of PCOS and lead to increase serum level of LH that due to hyperandrogenism.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Advanced Biotechnology and Experimental Therapeutics
Journal of Advanced Biotechnology and Experimental Therapeutics Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.90
自引率
0.00%
发文量
41
审稿时长
8 weeks
期刊最新文献
Assessment of antiproliferative and toxic effects of a peptide from Momordica dioica using in vitro and in vivo studies Investigation of the relationship between matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase with SARS CoV-2 infections Anti-inflammatory and anti-microbial activities of the phytochemicals isolated from various parts of broccoli wastes Epidemiological burden, risk factors, and recent therapeutic advances in chronic obstructive pulmonary disease The potentials of Pangi leaf extract for Aedes spp. mosquito control
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1