M. Moniruzzaman, M. Jinnah, Most Mostari, Shirmin Islam, M. Pramanik, Jui Biswas, Alam Imran, M. Uddin, M. Saleh, S. Zaman
{"title":"土传伯克氏菌MB-01对shobicron和垂直降解的效果","authors":"M. Moniruzzaman, M. Jinnah, Most Mostari, Shirmin Islam, M. Pramanik, Jui Biswas, Alam Imran, M. Uddin, M. Saleh, S. Zaman","doi":"10.5455/jabet.2022.d143","DOIUrl":null,"url":null,"abstract":"Pesticides are necessary in agriculture, yet their highly toxic ingredients harm the ecosystem. Due of their toxicity, uncontrolled releases of large quantities of pesticides pollute the environment and provide a larger health risk to plants, animals, and humans. Bacteria are capable of degrading such pollutants and saving our ecosystem. In this work, a bacterial strain was isolated from Shobicron and Vertimec-treated lady's finger soil using enrichment culture. The strain was identified as Burkholderia Cepacia-Like MB-01 based on morphological, physiological, and biochemical traits, as well as phylogenetic analysis of the 16SrRNA sequence. The bacterium grew best at 35 °C with a pH of 7. Furthermore, it was susceptible (S) to Cefepime and Penicillin but intermediate (I) resistant to Carbapenem and Tetracycline and resistant (R) to Ciprofloxacin, Kanamycin, and Gentamycin in an antibiotic sensitivity test. The rate of shobicron and vertimec degradation was measured over a five-day period using Mineral Salt (MS) medium. In its optimum growth condition, shobicron and vertimec degradation rates were determined to be around 76 % and 80 %, respectively. The isolated bacterial strain was found to be capable of detoxifying shobicron and vertimec in the experiment. As a result, the bacterial strain could be exploited as a possible shobicron and vertimec degrader for pesticide bioremediation.","PeriodicalId":36275,"journal":{"name":"Journal of Advanced Biotechnology and Experimental Therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Efficacy of soil-borne Burkholderia Cepacia-Like MB-01 strain for shobicron and vertimec degradation\",\"authors\":\"M. Moniruzzaman, M. Jinnah, Most Mostari, Shirmin Islam, M. Pramanik, Jui Biswas, Alam Imran, M. Uddin, M. Saleh, S. Zaman\",\"doi\":\"10.5455/jabet.2022.d143\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Pesticides are necessary in agriculture, yet their highly toxic ingredients harm the ecosystem. Due of their toxicity, uncontrolled releases of large quantities of pesticides pollute the environment and provide a larger health risk to plants, animals, and humans. Bacteria are capable of degrading such pollutants and saving our ecosystem. In this work, a bacterial strain was isolated from Shobicron and Vertimec-treated lady's finger soil using enrichment culture. The strain was identified as Burkholderia Cepacia-Like MB-01 based on morphological, physiological, and biochemical traits, as well as phylogenetic analysis of the 16SrRNA sequence. The bacterium grew best at 35 °C with a pH of 7. Furthermore, it was susceptible (S) to Cefepime and Penicillin but intermediate (I) resistant to Carbapenem and Tetracycline and resistant (R) to Ciprofloxacin, Kanamycin, and Gentamycin in an antibiotic sensitivity test. The rate of shobicron and vertimec degradation was measured over a five-day period using Mineral Salt (MS) medium. In its optimum growth condition, shobicron and vertimec degradation rates were determined to be around 76 % and 80 %, respectively. The isolated bacterial strain was found to be capable of detoxifying shobicron and vertimec in the experiment. As a result, the bacterial strain could be exploited as a possible shobicron and vertimec degrader for pesticide bioremediation.\",\"PeriodicalId\":36275,\"journal\":{\"name\":\"Journal of Advanced Biotechnology and Experimental Therapeutics\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Advanced Biotechnology and Experimental Therapeutics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/jabet.2022.d143\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Biotechnology and Experimental Therapeutics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/jabet.2022.d143","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Efficacy of soil-borne Burkholderia Cepacia-Like MB-01 strain for shobicron and vertimec degradation
Pesticides are necessary in agriculture, yet their highly toxic ingredients harm the ecosystem. Due of their toxicity, uncontrolled releases of large quantities of pesticides pollute the environment and provide a larger health risk to plants, animals, and humans. Bacteria are capable of degrading such pollutants and saving our ecosystem. In this work, a bacterial strain was isolated from Shobicron and Vertimec-treated lady's finger soil using enrichment culture. The strain was identified as Burkholderia Cepacia-Like MB-01 based on morphological, physiological, and biochemical traits, as well as phylogenetic analysis of the 16SrRNA sequence. The bacterium grew best at 35 °C with a pH of 7. Furthermore, it was susceptible (S) to Cefepime and Penicillin but intermediate (I) resistant to Carbapenem and Tetracycline and resistant (R) to Ciprofloxacin, Kanamycin, and Gentamycin in an antibiotic sensitivity test. The rate of shobicron and vertimec degradation was measured over a five-day period using Mineral Salt (MS) medium. In its optimum growth condition, shobicron and vertimec degradation rates were determined to be around 76 % and 80 %, respectively. The isolated bacterial strain was found to be capable of detoxifying shobicron and vertimec in the experiment. As a result, the bacterial strain could be exploited as a possible shobicron and vertimec degrader for pesticide bioremediation.