土传伯克氏菌MB-01对shobicron和垂直降解的效果

Q3 Biochemistry, Genetics and Molecular Biology Journal of Advanced Biotechnology and Experimental Therapeutics Pub Date : 2022-01-01 DOI:10.5455/jabet.2022.d143
M. Moniruzzaman, M. Jinnah, Most Mostari, Shirmin Islam, M. Pramanik, Jui Biswas, Alam Imran, M. Uddin, M. Saleh, S. Zaman
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引用次数: 0

摘要

农药在农业中是必需的,但其剧毒成分危害生态系统。由于其毒性,大量不受控制的农药排放会污染环境,并对植物、动物和人类带来更大的健康风险。细菌能够降解这些污染物,拯救我们的生态系统。在这项工作中,利用富集培养从Shobicron和vertime处理的女士手指土壤中分离出一株细菌。基于形态学、生理生化特征及16SrRNA序列的系统发育分析,该菌株被鉴定为牛头伯克霍尔德氏菌样MB-01。细菌在35℃、pH为7时生长最好。此外,在抗生素敏感性试验中,它对头孢吡肟和青霉素敏感(S),但对碳青霉烯和四环素耐药(I),对环丙沙星、卡那霉素和庆大霉素耐药(R)。用无矿盐(MS)培养基在5天的时间内测量了shobicron和垂直降解的速率。在其最佳生长条件下,shobicron和垂直降解率分别为76%和80%左右。实验结果表明,分离得到的菌株具有较强的解毒能力。研究结果表明,该菌株可作为农药生物修复的有效降解菌和垂直降解菌。
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Efficacy of soil-borne Burkholderia Cepacia-Like MB-01 strain for shobicron and vertimec degradation
Pesticides are necessary in agriculture, yet their highly toxic ingredients harm the ecosystem. Due of their toxicity, uncontrolled releases of large quantities of pesticides pollute the environment and provide a larger health risk to plants, animals, and humans. Bacteria are capable of degrading such pollutants and saving our ecosystem. In this work, a bacterial strain was isolated from Shobicron and Vertimec-treated lady's finger soil using enrichment culture. The strain was identified as Burkholderia Cepacia-Like MB-01 based on morphological, physiological, and biochemical traits, as well as phylogenetic analysis of the 16SrRNA sequence. The bacterium grew best at 35 °C with a pH of 7. Furthermore, it was susceptible (S) to Cefepime and Penicillin but intermediate (I) resistant to Carbapenem and Tetracycline and resistant (R) to Ciprofloxacin, Kanamycin, and Gentamycin in an antibiotic sensitivity test. The rate of shobicron and vertimec degradation was measured over a five-day period using Mineral Salt (MS) medium. In its optimum growth condition, shobicron and vertimec degradation rates were determined to be around 76 % and 80 %, respectively. The isolated bacterial strain was found to be capable of detoxifying shobicron and vertimec in the experiment. As a result, the bacterial strain could be exploited as a possible shobicron and vertimec degrader for pesticide bioremediation.
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来源期刊
Journal of Advanced Biotechnology and Experimental Therapeutics
Journal of Advanced Biotechnology and Experimental Therapeutics Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.90
自引率
0.00%
发文量
41
审稿时长
8 weeks
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