纳米氧化锌与二极管激光联合对结直肠癌细胞周期阻滞和细胞凋亡的细胞毒作用

Q3 Biochemistry, Genetics and Molecular Biology Journal of Advanced Biotechnology and Experimental Therapeutics Pub Date : 2023-01-01 DOI:10.5455/jabet.2023.d146
Chrakhan Rahman, Twana Mustafa
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引用次数: 0

摘要

全球癌症相关死亡的第四大致命原因是结直肠癌(CaCo2)。氧化锌纳米颗粒(ZnO-NPs)因其抗癌、抗糖尿病、抗菌和抗炎等特性而被广泛应用于生物医学领域。光动力治疗(PDT)通过光敏剂(ps)被光激活时产生的细胞毒性活性氧(ROS),从而导致癌细胞死亡,对恶性细胞产生选择性细胞毒性。研究了不同浓度和波长对CaCo2的细胞毒作用,采用联合治疗降低细胞株百分比对早期凋亡有明显影响(p值0.05)。综上所述,本研究支持联合治疗在体外对CaCo2具有细胞毒性,有可能成为一种有效的治疗药物。
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Cytotoxic effect of zinc oxide nanoparticle and diode laser combination in colorectal cancer in vitro via induction of cell cycle arrest and apoptosis
The fourth deadliest reason for cancer-related deaths worldwide was found to be colorectal cancer (CaCo2). Zinc oxide nanoparticles (ZnO-NPs) have been researched for biomedical applications due to their demonstrated anticancer, antidiabetic, antibacterial, and anti-inflammatory. by way of the cytotoxic reactive oxygen species (ROS) that are produced when photosensitizers (PSs) are activated by light, which in turn cause cancer cell death, photodynamic treatment (PDT) causes selective cytotoxicity to malignant cells. The cytotoxic effect of different concentrations and wavelengths was studied on CaCo2, and the use of combination therapy to reduce the percentage of cell lines had clear effects on early apoptosis (p-value<0.05). The half-maximal inhibitory concentration (IC50) for the cell was evaluated. The proper IC50 for CaCo2 was 20 ug/ml and the target of Diode laser power is 5 5 j/cm 2. In the G2 phase of the cell cycle, a lower percentage of cells relative to the S and G1 phases was significantly different when laser and ZnO2 were combined (p-value<0.05). Real-time Polyclonal chain reaction (PCR); significantly substantial variation in the use of a diode laser and ZnO2 to stop the spread of cancer by boosting the expression of the p53 gene (p-value<0000). Bax a central cell death regulator, was expressed significantly (p-value<0.05). Even so, utilizing the laser and ZnO2 together did not reveal any substantial differences in the Bcl2 gene (p-value>0.05). In conclusion, this study supported that combination therapy was cytotoxic to CaCo2 in vitro and there is a possibility of developing it as an effective therapeutic agent.
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来源期刊
Journal of Advanced Biotechnology and Experimental Therapeutics
Journal of Advanced Biotechnology and Experimental Therapeutics Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.90
自引率
0.00%
发文量
41
审稿时长
8 weeks
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