从伊朗马和狗身上分离的埃立克体物种的系统发育分析。

IF 2 3区农林科学 Q4 IMMUNOLOGY Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2023-10-24 DOI:10.1016/j.cimid.2023.102080

Siamak Molazadeh , Amir Tukmechi , Mojtaba Hadian , Bahram Dalir-Naghadeh

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引用次数: 0

摘要

本研究旨在确定埃立克体在伊朗马和狗中的流行率和系统发育分析。从伊朗五个不同省份的400只动物身上采集了血样，其中包括200匹马和200条狗。聚合酶链式反应（PCR）用于检测埃立克体。基于16S rRNA基因的扩增。采用半巢式PCR方法扩增dsb、TRP36和gltA基因。结果显示，4.5%的样本（3%的马和6%的狗）对埃立克体属呈阳性。最高的流行率出现在克尔曼和胡齐斯坦，而最低的流行率则出现在西阿塞拜疆、戈勒斯坦和马赞德兰。该研究表明，该国的狗和马的数量应被视为艾氏病流行病学的重要因素。基于dsb和TRP36基因的系统发育分析表明，流行种为犬E.canis和反刍E.反刍动物。

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Phylogenetic analysis of Ehrlichia species isolated from horses and dogs in Iran

This study aimed to determine the prevalence and phylogenetic analysis of Ehrlichia spp. in horses and dogs in Iran. Blood samples were collected from 400 animals, including 200 horses and 200 dogs, from five different provinces in Iran. Polymerase chain reaction (PCR) was used to detect Ehrlichia spp. based on amplification of the 16S rRNA gene. The semi-nested PCR method was used to amplify the dsb, TRP36, and gltA genes. The results showed that 4.5 % of the samples (3 % horses and 6 % dogs) were positive for Ehrlichia sp. The highest prevalence was observed in Kerman and Khuzestan, while the lowest was found in West Azerbaijan, Golestan, and Mazandaran. The study suggests that the populations of dogs and horses in the country should be considered important factors in the epidemiology of ehrlichiosis. Phylogenetic analysis based on the dsb and TRP36 genes revealed that the prevalent species were E. canis and E. ruminantium.

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来源期刊

Comparative Immunology Microbiology and Infectious Diseases 医学-免疫学

CiteScore

4.60

自引率

0.00%

发文量

102

审稿时长

40 days

期刊介绍： Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.