Lu Bai , Jayoung Paek , Yeseul Shin , Hongik Kim , Si Hyun Kim , Jeong Hwan Shin , Joong-Ki Kook , Young-Hyo Chang
{"title":"从猪粪便中分离出的梭形厌氧菌克氏镰刀菌（Fusibcillus kribianus gen.nov.，sp.nov.）的描述。","authors":"Lu Bai , Jayoung Paek , Yeseul Shin , Hongik Kim , Si Hyun Kim , Jeong Hwan Shin , Joong-Ki Kook , Young-Hyo Chang","doi":"10.1016/j.anaerobe.2023.102792","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>The family <em>Lachnospiraceae</em> is affiliated with the order Clostridiales and was originally contained within Clostridial cluster XIVa. The members of <em>Lachnospiraceae</em> inhabiting the gut comprise the chemoorganotrophic genera, generating sundry short-chain fatty acids to supply energy to the host, and are considered to be related to obesity and gut health.</p></div><div><h3>Methods</h3><p>The polyphasic taxonomic approach was used to characterize the isolate YH-rum2234<sup>T</sup>. A detailed metabolic analysis was conducted to compare the novel isolate with related strains within the family <em>Lachnospiraceae</em>.</p></div><div><h3>Results</h3><p>A fusiform, obligately anaerobic, Gram-stain-negative bacterium, YH-rum2234<sup>T</sup>, was isolated from pig feces. Analysis of the 16 S rRNA gene sequence revealed that the similarities between the isolate and the familiarly interrelated strain <em>Lientehia hominis</em> KCTC 25345<sup>T</sup> was 94.3 %. The average nucleotide identities and genome-to-genome distances of YH-rum2234<sup>T</sup> and its closely related strains were below 85.5 % and 32.5 %, respectively. The G + C content of the genomic DNA was 49.2 mol%. The main fatty acids were C<sub>16:0</sub>, C<sub>14:0</sub>, and C<sub>14:0</sub> DMA. The major polar lipids were aminophospholipids. The cell wall did not contain the peptidoglycan <em>meso</em>-diaminopimelic acid.</p></div><div><h3>Conclusion</h3><p>Given the chemotaxonomic, phenotypic, and phylogenetic properties, YH-rum2234<sup>T</sup> (=KCTC 25710<sup>T</sup> = DSMZ 116041<sup>T</sup>) represents a new genus and species in the family <em>Lachnospiraceae</em>. <em>Fusibacillus kribbianus</em> gen. nov., sp. nov. is the proposed name.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"84 ","pages":"Article 102792"},"PeriodicalIF":2.5000,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1075996423001014/pdfft?md5=82aac80a5f08a2ec78d355d908551856&pid=1-s2.0-S1075996423001014-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Description of Fusibacillus kribbianus gen. nov., sp. nov., a fusiform anaerobe isolated from pig feces\",\"authors\":\"Lu Bai , Jayoung Paek , Yeseul Shin , Hongik Kim , Si Hyun Kim , Jeong Hwan Shin , Joong-Ki Kook , Young-Hyo Chang\",\"doi\":\"10.1016/j.anaerobe.2023.102792\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>The family <em>Lachnospiraceae</em> is affiliated with the order Clostridiales and was originally contained within Clostridial cluster XIVa. The members of <em>Lachnospiraceae</em> inhabiting the gut comprise the chemoorganotrophic genera, generating sundry short-chain fatty acids to supply energy to the host, and are considered to be related to obesity and gut health.</p></div><div><h3>Methods</h3><p>The polyphasic taxonomic approach was used to characterize the isolate YH-rum2234<sup>T</sup>. A detailed metabolic analysis was conducted to compare the novel isolate with related strains within the family <em>Lachnospiraceae</em>.</p></div><div><h3>Results</h3><p>A fusiform, obligately anaerobic, Gram-stain-negative bacterium, YH-rum2234<sup>T</sup>, was isolated from pig feces. Analysis of the 16 S rRNA gene sequence revealed that the similarities between the isolate and the familiarly interrelated strain <em>Lientehia hominis</em> KCTC 25345<sup>T</sup> was 94.3 %. The average nucleotide identities and genome-to-genome distances of YH-rum2234<sup>T</sup> and its closely related strains were below 85.5 % and 32.5 %, respectively. The G + C content of the genomic DNA was 49.2 mol%. The main fatty acids were C<sub>16:0</sub>, C<sub>14:0</sub>, and C<sub>14:0</sub> DMA. The major polar lipids were aminophospholipids. The cell wall did not contain the peptidoglycan <em>meso</em>-diaminopimelic acid.</p></div><div><h3>Conclusion</h3><p>Given the chemotaxonomic, phenotypic, and phylogenetic properties, YH-rum2234<sup>T</sup> (=KCTC 25710<sup>T</sup> = DSMZ 116041<sup>T</sup>) represents a new genus and species in the family <em>Lachnospiraceae</em>. <em>Fusibacillus kribbianus</em> gen. nov., sp. nov. is the proposed name.</p></div>\",\"PeriodicalId\":8050,\"journal\":{\"name\":\"Anaerobe\",\"volume\":\"84 \",\"pages\":\"Article 102792\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-11-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1075996423001014/pdfft?md5=82aac80a5f08a2ec78d355d908551856&pid=1-s2.0-S1075996423001014-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anaerobe\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1075996423001014\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anaerobe","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1075996423001014","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Description of Fusibacillus kribbianus gen. nov., sp. nov., a fusiform anaerobe isolated from pig feces
Objective
The family Lachnospiraceae is affiliated with the order Clostridiales and was originally contained within Clostridial cluster XIVa. The members of Lachnospiraceae inhabiting the gut comprise the chemoorganotrophic genera, generating sundry short-chain fatty acids to supply energy to the host, and are considered to be related to obesity and gut health.
Methods
The polyphasic taxonomic approach was used to characterize the isolate YH-rum2234T. A detailed metabolic analysis was conducted to compare the novel isolate with related strains within the family Lachnospiraceae.
Results
A fusiform, obligately anaerobic, Gram-stain-negative bacterium, YH-rum2234T, was isolated from pig feces. Analysis of the 16 S rRNA gene sequence revealed that the similarities between the isolate and the familiarly interrelated strain Lientehia hominis KCTC 25345T was 94.3 %. The average nucleotide identities and genome-to-genome distances of YH-rum2234T and its closely related strains were below 85.5 % and 32.5 %, respectively. The G + C content of the genomic DNA was 49.2 mol%. The main fatty acids were C16:0, C14:0, and C14:0 DMA. The major polar lipids were aminophospholipids. The cell wall did not contain the peptidoglycan meso-diaminopimelic acid.
Conclusion
Given the chemotaxonomic, phenotypic, and phylogenetic properties, YH-rum2234T (=KCTC 25710T = DSMZ 116041T) represents a new genus and species in the family Lachnospiraceae. Fusibacillus kribbianus gen. nov., sp. nov. is the proposed name.
期刊介绍：
Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes.
Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.
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