AT-04磁场照射神经调控通过下行疼痛调节系统和阿片类镇痛改善神经性疼痛大鼠模型中的高痛症。

IF 3.6 4区 医学 Q3 CELL BIOLOGY Cellular and Molecular Neurobiology Pub Date : 2023-11-01 Epub Date: 2023-11-07 DOI:10.1007/s10571-023-01430-9
Tatsuro Kohno, Kaori Takaki, Kaori Kishita, Kazunori Mitsutake, Nozomu Tofuku, Iwao Kishita
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引用次数: 0

摘要

在我们之前的临床试验中,ait®(AT-04)是一种照射混合交变磁场(2 kHz和83.3 MHz)的设备,通过磁场照射神经调控已被证明对纤维肌痛和腰痛具有很高的疗效。本研究的目的是使用坐骨神经部分结扎(PSL)模型作为神经性疼痛的动物模型来阐明AT-04的潜在镇痛机制。将AT-04应用于PSL模型大鼠的痛觉过敏,并用von Frey法检测机械性痛觉异常,验证其镇痛作用。结果表明PSL模型大鼠的痛觉过敏有显著改善。我们还通过5-羟色胺和去甲肾上腺素受体拮抗剂的拮抗作用,研究了下行疼痛调节系统在AT-04镇痛作用中的作用。这些拮抗剂显著降低了AT-04对PSL模型大鼠疼痛的镇痛作用约50%。我们还在AT-04治疗期间使用微透析测量了PSL模型大鼠脊髓液中5-羟色胺和去甲肾上腺素的量。AT-04磁场照射后,两种单胺类物质均显著增加。此外,我们使用纳洛酮(阿片受体的主要拮抗剂)评估了阿片镇痛在AT-04镇痛效果中的作用,发现它显著拮抗了约60%的效果。因此,AT-04在PSL模型大鼠中的镇痛作用涉及内源性疼痛调节系统,包括下行疼痛调节系统和阿片类镇痛系统。
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Neuromodulation Through Magnetic Fields Irradiation with AT-04 Improves Hyperalgesia in a Rat Model of Neuropathic Pain via Descending Pain Modulatory Systems and Opioid Analgesia.

Neuromodulation through magnetic fields irradiation with ait® (AT-04), a device that irradiates a mixed alternating magnetic fields (2 kHz and 83.3 MHz), has been shown to have high efficacy for fibromyalgia and low back pain in our previous clinical trials. The aim of this study was to elucidate the underlying analgesic mechanism of the AT-04 using the partial sciatic nerve ligation (PSL) model as an animal model of neuropathic pain. AT-04 was applied to PSL model rats with hyperalgesia and its pain-improving effect was verified by examining mechanical allodynia using the von Frey method. The results demonstrated a significant improvement in hyperalgesia in PSL model rats. We also examined the involvement of descending pain modulatory systems in the analgesic effects of AT-04 using antagonism by serotonin and noradrenergic receptor antagonists. These antagonists significantly reduced the analgesic effect of AT-04 on pain in PSL model rats by approximately 50%. We also measured the amount of serotonin and noradrenaline in the spinal fluid of PSL model rats using microdialysis during AT-04 treatment. Both monoamines were significantly increased by magnetic fields irradiation with AT-04. Furthermore, we evaluated the involvement of opioid analgesia in the analgesic effects of AT-04 using naloxone, the main antagonist of the opioid receptor, and found that it significantly antagonized the effects by approximately 60%. Therefore, the analgesic effects of AT-04 in PSL model rats involve both the endogenous pain modulation systems, including the descending pain modulatory system and the opioid analgesic system.

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来源期刊
CiteScore
7.70
自引率
0.00%
发文量
137
审稿时长
4-8 weeks
期刊介绍: Cellular and Molecular Neurobiology publishes original research concerned with the analysis of neuronal and brain function at the cellular and subcellular levels. The journal offers timely, peer-reviewed articles that describe anatomic, genetic, physiologic, pharmacologic, and biochemical approaches to the study of neuronal function and the analysis of elementary mechanisms. Studies are presented on isolated mammalian tissues and intact animals, with investigations aimed at the molecular mechanisms or neuronal responses at the level of single cells. Cellular and Molecular Neurobiology also presents studies of the effects of neurons on other organ systems, such as analysis of the electrical or biochemical response to neurotransmitters or neurohormones on smooth muscle or gland cells.
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