Alessandra S. Dias , Anna Beatriz Rodrigues Gonçalves , Tatiana Lúcia Santos Nogueira , Dayse A. Silva
{"title":"从犯罪现场鉴定生物痕迹的分子方法的发展和验证","authors":"Alessandra S. Dias , Anna Beatriz Rodrigues Gonçalves , Tatiana Lúcia Santos Nogueira , Dayse A. Silva","doi":"10.1016/j.fsigss.2022.10.006","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to develop a methodology to identify biological fluids in sexual assault cases through mRNA markers. Biological fluid samples such as blood, saliva, and semen were collected from volunteers and submitted to RT-qPCR reactions with specific primers for the biomarkers HTN3 (saliva), ALAS (blood) andTGM4 (semen). The Melting (Tm) of each biological fluid was analyzed and the result inferred a high specificity capable of differentiating such traces. Biplex systems were generated to improve trace analysis in a single qPCR reaction.</p></div>","PeriodicalId":56262,"journal":{"name":"Forensic Science International: Genetics Supplement Series","volume":"8 ","pages":"Pages 125-127"},"PeriodicalIF":0.5000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1875176822000439/pdfft?md5=92d4ad749a56877c8f2dcc3aa887cc7e&pid=1-s2.0-S1875176822000439-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Development and validation of molecular methodologies for identification of biological traces from crime scenes\",\"authors\":\"Alessandra S. Dias , Anna Beatriz Rodrigues Gonçalves , Tatiana Lúcia Santos Nogueira , Dayse A. Silva\",\"doi\":\"10.1016/j.fsigss.2022.10.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to develop a methodology to identify biological fluids in sexual assault cases through mRNA markers. Biological fluid samples such as blood, saliva, and semen were collected from volunteers and submitted to RT-qPCR reactions with specific primers for the biomarkers HTN3 (saliva), ALAS (blood) andTGM4 (semen). The Melting (Tm) of each biological fluid was analyzed and the result inferred a high specificity capable of differentiating such traces. Biplex systems were generated to improve trace analysis in a single qPCR reaction.</p></div>\",\"PeriodicalId\":56262,\"journal\":{\"name\":\"Forensic Science International: Genetics Supplement Series\",\"volume\":\"8 \",\"pages\":\"Pages 125-127\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1875176822000439/pdfft?md5=92d4ad749a56877c8f2dcc3aa887cc7e&pid=1-s2.0-S1875176822000439-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Forensic Science International: Genetics Supplement Series\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1875176822000439\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic Science International: Genetics Supplement Series","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1875176822000439","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Development and validation of molecular methodologies for identification of biological traces from crime scenes
This study aimed to develop a methodology to identify biological fluids in sexual assault cases through mRNA markers. Biological fluid samples such as blood, saliva, and semen were collected from volunteers and submitted to RT-qPCR reactions with specific primers for the biomarkers HTN3 (saliva), ALAS (blood) andTGM4 (semen). The Melting (Tm) of each biological fluid was analyzed and the result inferred a high specificity capable of differentiating such traces. Biplex systems were generated to improve trace analysis in a single qPCR reaction.
期刊介绍：
The Journal of Forensic Science International Genetics Supplement Series is the perfect publication vehicle for the proceedings of a scientific symposium, commissioned thematic issues, or for disseminating a selection of invited articles. The Forensic Science International Genetics Supplement Series is part of a duo of publications on forensic genetics, published by Elsevier on behalf of the International Society for Forensic Genetics.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
