{"title":"在多物种生物膜模型中，负载氢氧化钙的聚（乳酸-乙醇酸）可生物降解纳米颗粒作为管内药物对抗内胚层致病微生物的功效。","authors":"Warat Leelapornpisid DDS, Piyaporn Wanwatanakul DDS, Thanisorn Mahatnirunkul BSc, MSc, PhD","doi":"10.1111/aej.12812","DOIUrl":null,"url":null,"abstract":"<p>This study aimed to evaluate the antimicrobial activity of calcium hydroxide-loaded poly(lactic-co-glycolic acid) nanoparticles (CH-loaded PLGA NPs) on multi-species biofilms. Human root blocks were prepared (<i>n</i> = 40), and multi-species suspensions of <i>Candida albicans</i>, <i>Enterococcus faecalis</i> and <i>Streptococcus gordonii</i> were incubated within the root canals for 21 days. Canals (<i>n</i> = 10/group) were then medicated with saline solution (negative control), chlorhexidine (positive control), calcium hydroxide and CH-loaded PLGA NPs for 7 days. Samples taken from the 0.1 mm root canal dentin were collected, and cell growth was detected by culture on BHI agar. The viable cell count of the Ca(OH)<sub>2</sub>, chlorhexidine gel and CH-loaded PLGA NPs group was significantly lower than the normal saline group (<i>p</i> < 0.001). CH-loaded PLGA NPs demonstrated a significant lower viable cell than Ca(OH)<sub>2</sub> (<i>p</i> < 0.001); it has potential as a medicament for endodontic therapy.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Efficacy of calcium hydroxide-loaded poly(lactic-co-glycolic acid) biodegradable nanoparticles as an intracanal medicament against endodontopathogenic microorganisms in a multi-species biofilm model\",\"authors\":\"Warat Leelapornpisid DDS, Piyaporn Wanwatanakul DDS, Thanisorn Mahatnirunkul BSc, MSc, PhD\",\"doi\":\"10.1111/aej.12812\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This study aimed to evaluate the antimicrobial activity of calcium hydroxide-loaded poly(lactic-co-glycolic acid) nanoparticles (CH-loaded PLGA NPs) on multi-species biofilms. Human root blocks were prepared (<i>n</i> = 40), and multi-species suspensions of <i>Candida albicans</i>, <i>Enterococcus faecalis</i> and <i>Streptococcus gordonii</i> were incubated within the root canals for 21 days. Canals (<i>n</i> = 10/group) were then medicated with saline solution (negative control), chlorhexidine (positive control), calcium hydroxide and CH-loaded PLGA NPs for 7 days. Samples taken from the 0.1 mm root canal dentin were collected, and cell growth was detected by culture on BHI agar. The viable cell count of the Ca(OH)<sub>2</sub>, chlorhexidine gel and CH-loaded PLGA NPs group was significantly lower than the normal saline group (<i>p</i> < 0.001). CH-loaded PLGA NPs demonstrated a significant lower viable cell than Ca(OH)<sub>2</sub> (<i>p</i> < 0.001); it has potential as a medicament for endodontic therapy.</p>\",\"PeriodicalId\":55581,\"journal\":{\"name\":\"Australian Endodontic Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2023-11-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Australian Endodontic Journal\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/aej.12812\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australian Endodontic Journal","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/aej.12812","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Efficacy of calcium hydroxide-loaded poly(lactic-co-glycolic acid) biodegradable nanoparticles as an intracanal medicament against endodontopathogenic microorganisms in a multi-species biofilm model
This study aimed to evaluate the antimicrobial activity of calcium hydroxide-loaded poly(lactic-co-glycolic acid) nanoparticles (CH-loaded PLGA NPs) on multi-species biofilms. Human root blocks were prepared (n = 40), and multi-species suspensions of Candida albicans, Enterococcus faecalis and Streptococcus gordonii were incubated within the root canals for 21 days. Canals (n = 10/group) were then medicated with saline solution (negative control), chlorhexidine (positive control), calcium hydroxide and CH-loaded PLGA NPs for 7 days. Samples taken from the 0.1 mm root canal dentin were collected, and cell growth was detected by culture on BHI agar. The viable cell count of the Ca(OH)2, chlorhexidine gel and CH-loaded PLGA NPs group was significantly lower than the normal saline group (p < 0.001). CH-loaded PLGA NPs demonstrated a significant lower viable cell than Ca(OH)2 (p < 0.001); it has potential as a medicament for endodontic therapy.
期刊介绍：
The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues.
The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses.
The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.
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