Chun-Yan Zhang, Shun Xu, Lei Yu, Yi-Wen Nie, Si-Bo Li, Jian-Yong Zhu
{"title":"大戟中的Lathyrane二萜类化合物诱导人增生性瘢痕细胞的细胞周期停滞和凋亡。","authors":"Chun-Yan Zhang, Shun Xu, Lei Yu, Yi-Wen Nie, Si-Bo Li, Jian-Yong Zhu","doi":"10.1080/14786419.2023.2280736","DOIUrl":null,"url":null,"abstract":"<p><p>One new lathyrane-type diterpenoid, euphlathin A (<b>1</b>), and 11 known analogues (<b>2-12</b>), were isolated from the fruits of <i>Euphorbia lathyris</i>. Their structures were elucidated by spectroscopic data. The absolute configurations of <b>1</b> were established by single-crystal X-ray crystallography. All diterpenoids (<b>1-12</b>) were evaluated for antiproliferative activity against the human hypertrophic scar (HTS) cells. Compound <b>1</b> exhibited significantly against HTS cells growth with an IC<sub>50</sub> value of 6.33 μM. Morphological features of apoptosis were evaluated in <b>1</b>-treated HTS cells. Wound healing assays indicated that <b>1</b> significantly inhibited the migration of HTS at 24 h and 48 h. Compound <b>1</b> effectively induced apoptosis of HTS, which was associated with G<sub>2</sub>/M or S phase cell cycle arrest. Flow cytometric analysis showed that the treatment by <b>1</b> significantly induced HTS cell apoptosis in a dose-dependent manner. Overall, euphlathin A (<b>1</b>) has the potential to be a therapeutic agent for the treatment of hyperplastic scar therapy.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"4181-4190"},"PeriodicalIF":1.9000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Lathyrane diterpenoids from <i>Euphorbia lathyris</i> induce cell-cycle arrest and apoptosis in human hypertrophic scar cells.\",\"authors\":\"Chun-Yan Zhang, Shun Xu, Lei Yu, Yi-Wen Nie, Si-Bo Li, Jian-Yong Zhu\",\"doi\":\"10.1080/14786419.2023.2280736\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>One new lathyrane-type diterpenoid, euphlathin A (<b>1</b>), and 11 known analogues (<b>2-12</b>), were isolated from the fruits of <i>Euphorbia lathyris</i>. Their structures were elucidated by spectroscopic data. The absolute configurations of <b>1</b> were established by single-crystal X-ray crystallography. All diterpenoids (<b>1-12</b>) were evaluated for antiproliferative activity against the human hypertrophic scar (HTS) cells. Compound <b>1</b> exhibited significantly against HTS cells growth with an IC<sub>50</sub> value of 6.33 μM. Morphological features of apoptosis were evaluated in <b>1</b>-treated HTS cells. Wound healing assays indicated that <b>1</b> significantly inhibited the migration of HTS at 24 h and 48 h. Compound <b>1</b> effectively induced apoptosis of HTS, which was associated with G<sub>2</sub>/M or S phase cell cycle arrest. Flow cytometric analysis showed that the treatment by <b>1</b> significantly induced HTS cell apoptosis in a dose-dependent manner. Overall, euphlathin A (<b>1</b>) has the potential to be a therapeutic agent for the treatment of hyperplastic scar therapy.</p>\",\"PeriodicalId\":18990,\"journal\":{\"name\":\"Natural Product Research\",\"volume\":\" \",\"pages\":\"4181-4190\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Natural Product Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1080/14786419.2023.2280736\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/11/10 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/14786419.2023.2280736","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/10 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Lathyrane diterpenoids from Euphorbia lathyris induce cell-cycle arrest and apoptosis in human hypertrophic scar cells.
One new lathyrane-type diterpenoid, euphlathin A (1), and 11 known analogues (2-12), were isolated from the fruits of Euphorbia lathyris. Their structures were elucidated by spectroscopic data. The absolute configurations of 1 were established by single-crystal X-ray crystallography. All diterpenoids (1-12) were evaluated for antiproliferative activity against the human hypertrophic scar (HTS) cells. Compound 1 exhibited significantly against HTS cells growth with an IC50 value of 6.33 μM. Morphological features of apoptosis were evaluated in 1-treated HTS cells. Wound healing assays indicated that 1 significantly inhibited the migration of HTS at 24 h and 48 h. Compound 1 effectively induced apoptosis of HTS, which was associated with G2/M or S phase cell cycle arrest. Flow cytometric analysis showed that the treatment by 1 significantly induced HTS cell apoptosis in a dose-dependent manner. Overall, euphlathin A (1) has the potential to be a therapeutic agent for the treatment of hyperplastic scar therapy.
期刊介绍：
The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds.
The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal.
Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section.
All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
