绿茶提取物对生鸡肉中金黄色葡萄球菌和肺炎克雷伯菌的抑制作用研究

Meat Research Pub Date : 2023-02-28 DOI:10.55002/mr.3.1.45
KF Kaderi, M. Islam, T. Sarker, SS Islam, D. Mondol, M. Faruk, G. Biswas
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引用次数: 0

摘要

研究了绿茶提取物对生鸡肉中金黄色葡萄球菌和肺炎克雷伯菌的抑制作用。试验采用完全随机设计(CRD),共6个处理,3个重复。采用滴板法测定其杀菌活性。结果表明,绿茶提取物(GTE)的添加降低了生鸡肉中的微生物数量。在10-3稀释系数(DF)下,T5菌落形成单位/ml (CFU ml-1)最低。不同治疗间金黄色葡萄球菌和肺炎克雷伯菌的CFU ml-1差异极显著(p<0.001),即GTE对肺炎克雷伯菌和金黄色葡萄球菌有显著影响(p<0.001)。TVC对生鸡肉中金黄色葡萄球菌的最大检出量为14×105 CFU ml-1, T5中最小检出量为4×104 CFU ml-1。肺炎克雷伯菌也有类似的趋势。GTE对金黄色葡萄球菌和克雷伯氏菌均有极显著的杀灭效果(p<0.001)。GTE对表皮葡萄球菌、沙门氏菌、志贺氏菌和大肠杆菌均有极显著(p<0.01)的杀灭效果。综上所述,添加5 ml GTE可降低生鸡的微生物数量。
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Study on the Efficacy of Green Tea Extract on Staphylococcus aureus and Klebsiella pneumonia of Raw Chicken Meat
We investigated the efficacy of green tea extract on Staphylococcus aureus and Klebsiella pneumonia of raw chicken meat. The experiment was conducted using complete randomized design (CRD) having six (6) treatments with three (3) replications. The germicidal activity was determined using the drop plate method. The results indicated that the addition of green tea extract (GTE) decreased microbial population in raw chicken meat. The lowest colony forming unit/ml (CFU ml-1) was found in T5 in 10-3 dilution factor (DF). There was a highly significant difference (p<0.001) in CFU ml-1of Staphylococcus aureus and Klebsiella pneumonia among different treatments i.e., GTE has significant effect (p<0.001) on Klebsiella pneumoniae and Staphylococcus aureus. The maximum number of Staphylococcus aureus in raw chicken meat was 14×105 CFU ml-1in T0 using TVC, while minimum was 4×104 CFU ml-1 in T5. Similar trend was also found in Klebsiella pneumoniae. GTE also showed highly significant (p<0.001) germicidal efficacy on both of Staphylococcus aureus and Klebsiell. The GTE also showed significant (p<0.01) germicidal efficacy on Staphylococcus epidermidis, Salmonella spp., Shigella spp. and E. coli. Considering the results obtained, it might be concluded that addition of 5 ml GTE reduce microbial population of raw chicken.
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