Phenotypic and Molecular Investigation of Streptococcus pneumoniae Hemolysin

The current study aimed to detect S.pneumoniae , which is one of the gram-positive bacteria that gives partial α-hemolysis on blood agar media, as well as has the ability to complete blood hemolysis (beta)-hemolysis under anaerobic conditions. Fifty sputum specimens were collected from patients in Ibn sena and Al Salam hospitals in Mosul/ Iraq from august 2021 to March 2022. Five isolates were diagnosed as S.pneumoniae which exhibited an ability to produce hemolysin when phenotypically investigated. Polymerase chain reaction (PCR) technique was used to detect hlys gene Electrophoresis results showed four bands (80% of isolates) with a molecular size of 296 bp the PCR product were then sequenced.