三种埃及食用菌的生物活性成分及体外抗氧化能力、抗菌性能和细胞毒性的研究

Ghada A. Youssef, Marwa M. Naguib, Doha M. Beltagy
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引用次数: 0

摘要

许多食用菌已被评价为具有各种活性的新的治疗选择。本研究主要研究了三种食用菌水提液的抗氧化、抗菌和细胞毒性作用:双孢蘑菇(Agaricus bisporus)、珍珠平菇(Pleurotus ostreatus)和凤凰菇(Pleurotus pulmonarius)。提取液的生化分析显示,总碳水化合物、蛋白质、酚类物质和类黄酮含量存在显著差异。采用2,2-二苯基-1-苦味基-水合肼(DPPH)自由基清除法测定三种提取物的抗氧化活性。平菇具有潜在的抗氧化能力,IC50值最低(13.26µg/mL)。对5种对照菌均有不同程度的抑菌活性,最低抑菌浓度(MIC)为28 ~ 89 mg/ml。双孢蘑菇(Agaricus bisporus)对EAC细胞具有最高的细胞毒性,细胞存活率为68%,对人乳腺癌细胞株(MCF-7)具有抑制作用。
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Bioactive compounds and in vitro evaluation of antioxidant capacities, antimicrobial properties, and effectiveness of cytotoxicity of three edible mushrooms from Egypt
Many edible mushrooms have been evaluated as new therapeutic alternatives with various activities. We focused this work to estimate the antioxidant, antimicrobial and cytotoxicity effects of three edible aqueous mushroom extracts: Agaricus bisporus (white button mushroom), Pleurotus ostreatus (pearl oyster mushroom), and Pleurotus pulmonarius (Phoenix mushroom). The biochemical analysis of extracts revealed marked variations for total carbohydrate, protein, phenolic, and flavonoid contents. The three extracts were tested for antioxidant activities using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay. Pleurotus ostreatus had a potential antioxidant capacity with lowest IC50 value (13.26 µg/mL). Distinct variable degrees of antimicrobial activity were detected against five reference microbial strains, exerting the minimum inhibitory concentration (MIC) ranging from 28 to 89 mg/ml. Agaricus bisporus exhibited the highest cytotoxicity against EAC cells with cell viability 68% and showed an evidenced inhibitory action against human breast cancer cell lines (MCF-7) based on Sulforhodamine B (SRB) assay.
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