三种埃及食用菌的生物活性成分及体外抗氧化能力、抗菌性能和细胞毒性的研究

Ghada A. Youssef, Marwa M. Naguib, Doha M. Beltagy
{"title":"三种埃及食用菌的生物活性成分及体外抗氧化能力、抗菌性能和细胞毒性的研究","authors":"Ghada A. Youssef, Marwa M. Naguib, Doha M. Beltagy","doi":"10.53771/ijlsra.2023.4.2.0051","DOIUrl":null,"url":null,"abstract":"Many edible mushrooms have been evaluated as new therapeutic alternatives with various activities. We focused this work to estimate the antioxidant, antimicrobial and cytotoxicity effects of three edible aqueous mushroom extracts: Agaricus bisporus (white button mushroom), Pleurotus ostreatus (pearl oyster mushroom), and Pleurotus pulmonarius (Phoenix mushroom). The biochemical analysis of extracts revealed marked variations for total carbohydrate, protein, phenolic, and flavonoid contents. The three extracts were tested for antioxidant activities using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay. Pleurotus ostreatus had a potential antioxidant capacity with lowest IC50 value (13.26 µg/mL). Distinct variable degrees of antimicrobial activity were detected against five reference microbial strains, exerting the minimum inhibitory concentration (MIC) ranging from 28 to 89 mg/ml. Agaricus bisporus exhibited the highest cytotoxicity against EAC cells with cell viability 68% and showed an evidenced inhibitory action against human breast cancer cell lines (MCF-7) based on Sulforhodamine B (SRB) assay.","PeriodicalId":14144,"journal":{"name":"International Journal of Life Science Research Archive","volume":"22 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioactive compounds and in vitro evaluation of antioxidant capacities, antimicrobial properties, and effectiveness of cytotoxicity of three edible mushrooms from Egypt\",\"authors\":\"Ghada A. Youssef, Marwa M. Naguib, Doha M. Beltagy\",\"doi\":\"10.53771/ijlsra.2023.4.2.0051\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Many edible mushrooms have been evaluated as new therapeutic alternatives with various activities. We focused this work to estimate the antioxidant, antimicrobial and cytotoxicity effects of three edible aqueous mushroom extracts: Agaricus bisporus (white button mushroom), Pleurotus ostreatus (pearl oyster mushroom), and Pleurotus pulmonarius (Phoenix mushroom). The biochemical analysis of extracts revealed marked variations for total carbohydrate, protein, phenolic, and flavonoid contents. The three extracts were tested for antioxidant activities using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay. Pleurotus ostreatus had a potential antioxidant capacity with lowest IC50 value (13.26 µg/mL). Distinct variable degrees of antimicrobial activity were detected against five reference microbial strains, exerting the minimum inhibitory concentration (MIC) ranging from 28 to 89 mg/ml. Agaricus bisporus exhibited the highest cytotoxicity against EAC cells with cell viability 68% and showed an evidenced inhibitory action against human breast cancer cell lines (MCF-7) based on Sulforhodamine B (SRB) assay.\",\"PeriodicalId\":14144,\"journal\":{\"name\":\"International Journal of Life Science Research Archive\",\"volume\":\"22 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Life Science Research Archive\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.53771/ijlsra.2023.4.2.0051\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Life Science Research Archive","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.53771/ijlsra.2023.4.2.0051","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

许多食用菌已被评价为具有各种活性的新的治疗选择。本研究主要研究了三种食用菌水提液的抗氧化、抗菌和细胞毒性作用:双孢蘑菇(Agaricus bisporus)、珍珠平菇(Pleurotus ostreatus)和凤凰菇(Pleurotus pulmonarius)。提取液的生化分析显示,总碳水化合物、蛋白质、酚类物质和类黄酮含量存在显著差异。采用2,2-二苯基-1-苦味基-水合肼(DPPH)自由基清除法测定三种提取物的抗氧化活性。平菇具有潜在的抗氧化能力,IC50值最低(13.26µg/mL)。对5种对照菌均有不同程度的抑菌活性,最低抑菌浓度(MIC)为28 ~ 89 mg/ml。双孢蘑菇(Agaricus bisporus)对EAC细胞具有最高的细胞毒性,细胞存活率为68%,对人乳腺癌细胞株(MCF-7)具有抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Bioactive compounds and in vitro evaluation of antioxidant capacities, antimicrobial properties, and effectiveness of cytotoxicity of three edible mushrooms from Egypt
Many edible mushrooms have been evaluated as new therapeutic alternatives with various activities. We focused this work to estimate the antioxidant, antimicrobial and cytotoxicity effects of three edible aqueous mushroom extracts: Agaricus bisporus (white button mushroom), Pleurotus ostreatus (pearl oyster mushroom), and Pleurotus pulmonarius (Phoenix mushroom). The biochemical analysis of extracts revealed marked variations for total carbohydrate, protein, phenolic, and flavonoid contents. The three extracts were tested for antioxidant activities using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay. Pleurotus ostreatus had a potential antioxidant capacity with lowest IC50 value (13.26 µg/mL). Distinct variable degrees of antimicrobial activity were detected against five reference microbial strains, exerting the minimum inhibitory concentration (MIC) ranging from 28 to 89 mg/ml. Agaricus bisporus exhibited the highest cytotoxicity against EAC cells with cell viability 68% and showed an evidenced inhibitory action against human breast cancer cell lines (MCF-7) based on Sulforhodamine B (SRB) assay.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Assessment of the effects of physiological development of cocoa (Theobroma cacao l.) explant on somatic embryogenesis Tinea capitis due to Microsporum canis in the ‎university hospital Hassan II of fez: ‎Epidemiological and mycological ‎profile The challenges of installing Picture Archiving and Communication Systems (PACS) in a low- or poor-income resource nation: Our experience in a teaching hospital in Sub-Saharan Africa Utilization of maggot (Heermetia illucens) as raw material for feed on the physical quality of fish feed Classical and molecular detection methods in aquatic environmental virology for waterborne diseases outbreaks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1