Y. Matsubara, Hiroyasu Kumamoto, Y. Iizuka, T. Murakami, K. Okamoto, H. Miyake, K. Yokoi
{"title":"柑桔皮中黄酮类苷的结构及降压作用","authors":"Y. Matsubara, Hiroyasu Kumamoto, Y. Iizuka, T. Murakami, K. Okamoto, H. Miyake, K. Yokoi","doi":"10.1271/BBB1961.49.909","DOIUrl":null,"url":null,"abstract":"An attempt was made to isolate hypotensive substances from a hot water extract of Citrus unshiu. Six flavonoid glycosides were isolated by repeated chromatography and gel filtration after extraction with butanol and treatment with lead subacetate. Their structures were established to be limocitrin 3-β-d-glucose (1), limocitrin-3-α-l-rhamnose (2), 3,6-di-C-glucosylapigenin (3), narirutin (4), rutin (5) and narcissin (6) by UV, 1-NMR and 13C-NMR spectroscopy and sugar analysis. Each component was intravenously injected into SHR-SP rats (1 mg/100g body weight), and 3 and 5 were found to lower their blood pressure.Among these compounds, 2 and 3 were a new flavonol and flavone glycoside, respectively.","PeriodicalId":7729,"journal":{"name":"Agricultural and biological chemistry","volume":"11 15 1","pages":"909-914"},"PeriodicalIF":0.0000,"publicationDate":"2014-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"55","resultStr":"{\"title\":\"Structure and Hypotensive Effect of Flavonoid Glycosides in Citrus unshiu Peelings\",\"authors\":\"Y. Matsubara, Hiroyasu Kumamoto, Y. Iizuka, T. Murakami, K. Okamoto, H. Miyake, K. Yokoi\",\"doi\":\"10.1271/BBB1961.49.909\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An attempt was made to isolate hypotensive substances from a hot water extract of Citrus unshiu. Six flavonoid glycosides were isolated by repeated chromatography and gel filtration after extraction with butanol and treatment with lead subacetate. Their structures were established to be limocitrin 3-β-d-glucose (1), limocitrin-3-α-l-rhamnose (2), 3,6-di-C-glucosylapigenin (3), narirutin (4), rutin (5) and narcissin (6) by UV, 1-NMR and 13C-NMR spectroscopy and sugar analysis. Each component was intravenously injected into SHR-SP rats (1 mg/100g body weight), and 3 and 5 were found to lower their blood pressure.Among these compounds, 2 and 3 were a new flavonol and flavone glycoside, respectively.\",\"PeriodicalId\":7729,\"journal\":{\"name\":\"Agricultural and biological chemistry\",\"volume\":\"11 15 1\",\"pages\":\"909-914\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"55\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural and biological chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1271/BBB1961.49.909\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural and biological chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1271/BBB1961.49.909","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 55
摘要
本文试图从青柑热水提取液中分离出降压物质。经丁醇提取、亚乙酸铅处理，经反复层析和凝胶过滤分离得到6种黄酮类苷。通过紫外光谱、1- nmr、13C-NMR和糖谱分析，确定了它们的结构分别为limmocitrin 3-β-d-葡萄糖(1)、limmocitrin -3-α-l-鼠李糖(2)、3,6-di- c -glucosylapigenin(3)、narirutin(4)、芦丁(5)和水仙素(6)。将各组分(1 mg/100g体重)静脉注射到SHR-SP大鼠体内，发现其中3和5具有降低血压的作用。其中化合物2和3分别为新发现的黄酮醇和黄酮苷。
Structure and Hypotensive Effect of Flavonoid Glycosides in Citrus unshiu Peelings
An attempt was made to isolate hypotensive substances from a hot water extract of Citrus unshiu. Six flavonoid glycosides were isolated by repeated chromatography and gel filtration after extraction with butanol and treatment with lead subacetate. Their structures were established to be limocitrin 3-β-d-glucose (1), limocitrin-3-α-l-rhamnose (2), 3,6-di-C-glucosylapigenin (3), narirutin (4), rutin (5) and narcissin (6) by UV, 1-NMR and 13C-NMR spectroscopy and sugar analysis. Each component was intravenously injected into SHR-SP rats (1 mg/100g body weight), and 3 and 5 were found to lower their blood pressure.Among these compounds, 2 and 3 were a new flavonol and flavone glycoside, respectively.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
