菲律宾森林木本藤本植物化学成分、总酚含量、抗氧化和抗菌活性的评价

Q3 Multidisciplinary Philippine Journal of Science Pub Date : 2023-04-24 DOI:10.56899/152.03.25
Kim Wilmer Balagot, Kimberly Delica, R. Lapuz, Rowena Ramos, Grace Rowena Bisana
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引用次数: 0

摘要

木本藤本植物是草本植物,它们利用不同的附着结构从附近的树木那里寻找垂直的支撑物。它们通常用于手工艺目的,但也有报道称它们被用于药用。在此基础上,研究了8种森林木本藤本植物的植物化学成分、总酚含量、抗氧化和抗菌活性。这些是马钱子小丹斯特。(" balakbakin ");Merr (" albotra "), Merremia peltata (L.)稳定。(“bulakan”),Hypserpa nitida Miers ex Benth(“lalapau”),Symphorema luzonicum (Blanco) f - vill。(“mulawing- bagging”),Dicranopteris linearis (Burm。f.)(“tilob”);Leenh。(kamagsa brown)和Entada phaseoloides (L.)Merr(“gugo”),这些都是原产于菲律宾的。结果表明,黄芪的提取率最高,分别为6.28±0.39%和6.26±0.77%。植物化学筛选表明,粗提物中含有不同的植物化学物质,如生物碱、类固醇、萜类和单宁。木本藤本植物乙醇提取物的总酚含量在9.51 ~ 640.24 mg GAE/g之间,其中半蜡木和芦杉的总酚含量最高,分别为640.24±7.13和398.94±20.08 mg GAE/g。此外,DPPH结果表明,线状木霉、芦竹霉、相色木霉和半蜡木霉具有较强的抗氧化活性,IC50范围为5.99 ~ 17.88 μg/mL,均高于丁基羟基甲苯(BHT)标准(28.47 μg/mL IC50)。褐皮霉、黄酮类、黑螺旋藻和S. minor的抗氧化活性在40.61 ~ 78.65 μg/mL之间。相似的FRAP测定结果表明,芦花草的抗氧化活性最高,为2.68±0.07 μmol TE/g干提取物,而山楂和黑荆的抗氧化活性最低,分别为0.43 μmol TE/g干提取物±0.03和0.41±0.02 μmol TE/g干提取物。采用孔扩散法对金黄色葡萄球菌进行抑菌试验,结果表明,金黄色葡萄球菌对芦花葡萄醇提物和半蜡葡萄醇提物的生长敏感,抑菌带分别为21.3±1.4 mm和20.1±2.5 mm。总体而言,结果表明木本藤本植物含有具有良好抗氧化和抗菌特性的植物化学物质。这些结果可作为木本藤生物活性成分纯化的基础。
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Evaluation of Phytochemicals, Total Phenolic Content, Antioxidant, and Antimicrobial Activities of Philippine Forest Woody Vines
Woody vines are herbaceous plants that use different clinging structures to find vertical support from nearby trees. They are commonly used for handicraft purposes but there were reports that they were used for medicinal applications. With this, the stems of eight forest woody vine species were evaluated for their phytochemical components, total phenolic content, antioxidant, and antimicrobial activities. These were Strychnos minor Denrst. (“balakbakin”), Arcangelisia flava (L.) Merr (“albotra”), Merremia peltata (L.) Merr. (“bulakan”), Hypserpa nitida Miers ex Benth (“lalapau”), Symphorema luzonicum (Blanco) F.-Vill. (“mulawing-baging”), Dicranopteris linearis (Burm. f.) (“tilob”), Connarus semidecandrus (Zoll.) Leenh. (“kamagsa brown”), and Entada phaseoloides (L.) Merr (“gugo”), which are all native to the Philippines. Results showed that S. minor and S. luzonicum had the highest extraction yield of 6.28 ± 0.39% and 6.26 ± 0.77%, respectively. Phytochemical screening revealed that the crude extracts contained different phytochemicals such as alkaloids, steroids, terpenoids, and tannins. For total phenolic content, ethanolic extracts of woody vines ranged from 9.51–640.24 mg GAE/g dried extract with C. semidecandrus and S. luzonicum were found to have higher amounts among forest woody vines with values of 640.24 ± 7.13 and 398.94 ± 20.08 mg GAE/g dried extract, respectively. Moreover, DPPH results showed that D. linearis, S. luzonicum, E. phaseoloides, and C. semidecandrus had strong antioxidant activities, which ranged from 5.99–17.88 μg/mL IC50 and were higher than the butylated hydroxytoluene (BHT) standard (28.47 μg/mL IC50). On the other hand, M. peltata, A. flava, H. nitida, and S. minor antioxidant activity ranged from 40.61–78.65 μg/mL IC50. Similar results of FRAP assay showed that S. luzonicum had the highest antioxidant activity with 2.68 ± 0.07 μmol TE/g dried extract, whereas S. minor and H. nitida had the lowest antioxidant activity with 0.43 μmol TE/g dried extract ± 0.03 and 0.41 ± 0.02 μmol TE/g dried extract, respectively. Among the tested microorganisms for antimicrobial assay (well diffusion method), the growth of S. aureus was susceptible to the ethanolic extracts of S. luzonicum and C. Semidecandrus with inhibition zones of 21.3 ± 1.4 and 20.1 ± 2.5 mm, respectively. Overall, results show that woody vines contain phytochemicals with promising antioxidant and antimicrobial properties. These results could be used as a baseline in the purification of woody vines’ bioactive components.
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来源期刊
Philippine Journal of Science
Philippine Journal of Science Multidisciplinary-Multidisciplinary
CiteScore
1.20
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0.00%
发文量
55
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